Simultaneous determination of five flavonoids components in different parts of Callistephus chinensis by HPLC / 中草药

Objective: To establish an HPLC method for the simultaneous determination of five flavonnid components in different parts of Callistephus chinensis. Methods: The chromatographic separation was performed on an XUnion C18 column (250 mm × 4.6 mm, 5 μm) with a gradient of acetonitrile and water, at a flow rate of 1 mL/min, and detected at 210 nm. Results: The calibration curve was linear within 2.5-200 mg/L for hyperoside, apigenin-7-O-β-D-glucopyranoside, luteolin, apigenin, and kaempferol, respectively, with the correlation r > 0.999 0. The extraction recoveries varied from 95% to 105%. Conclusion: The method is accurate and selective, and can be used for the quality control of different parts of C. chinensis.