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1.
Chinese Journal of Stomatology ; (12): 561-563, 2007.
Artigo em Chinês | WPRIM | ID: wpr-359694

RESUMO

<p><b>OBJECTIVE</b>To investigate the relationship between muscle segment homeobox gene-1 (MSX1) and the genetic susceptibility of nonsyndromic cleft lip and palate (NSCLP) in Hunan Hans.</p><p><b>METHODS</b>One microsatellite DNA marker CA repeat in MSX1 intron region was used as genetic marker. The genotypes of 387 members in 129 NSCLP nuclear family trios were analyzed by polymerase chain reaction (PCR) and denaturing polyacrylamide gel electrophoresis. Then transmission disequilibrium test (TDT) and Logistic regression analysis were used to conduct association analysis.</p><p><b>RESULTS</b>TDT analysis confirmed that CA4 allele in CL/P and CPO groups preferentially transmitted to the affected offspring (P = 0.018, P = 0.041). Logistic regression analysis indicated that the recessive model of inheritance was supported, and CA4 itself or CA4 acting as a marker for a disease allele or haplotype was inherited in a recessive fashion (P = 0.009).</p><p><b>CONCLUSIONS</b>MSX1 gene is associated with NSCLP, and MSX1 gene may be directly involved either in the etiology of NSCLP or in linkage disequilibrium with disease-predisposing sites.</p>


Assuntos
Humanos , Povo Asiático , Fenda Labial , Genética , Fissura Palatina , Genética , Marcadores Genéticos , Genética , Genótipo , Desequilíbrio de Ligação , Modelos Logísticos , Fator de Transcrição MSX1 , Genética , Repetições de Microssatélites , Genética , Linhagem
2.
Chinese Journal of Medical Genetics ; (6): 325-327, 2007.
Artigo em Chinês | WPRIM | ID: wpr-247324

RESUMO

<p><b>OBJECTIVE</b>To investigate muscle segment homeobox 1 (MSX1) microsatellite marker distribution and the relationship between MSX1 gene and the genetic susceptibility of nonsyndromic cleft lip and palate (NSCLP) in Hunan Hans.</p><p><b>METHODS</b>One microsatellite DNA marker CA repeat in MSX1 intron region was used as genetic markers. The genotypes of 129 patients with NSCLP and 108 controls were analyzed by the techniques of polymerase chain reaction (PCR) and denaturing polyacrylamide gel electrophoresis (PAGE). Then case-control study was used to conduct association analysis.</p><p><b>RESULTS</b>The allele frequencies of the CA repeat microsatellite DNA in Hunan Han normal population were in good agreement with Hardy-Weinberg equilibrium. The polymorphism information content and heterozygosity of CA repeat microsatellite DNA were 0.50 and 0.50 respectively. The allele CA4 frequency in CL/P and CPO group was significantly higher than that of normal controls (P<0.05). The genotype CA4,4 frequency was significantly higher in CL/P and CPO group than that in normal controls (P<0.05).</p><p><b>CONCLUSION</b>The microsatellite DNA marker CA repeat in MSX1 is a good genetic marker. MSX1 gene is significantly associated with NSCLP in Hunan Hans.</p>


Assuntos
Humanos , Sequência de Bases , China , Etnologia , Fenda Labial , Genética , Fissura Palatina , Genética , Etnicidade , Genética , Frequência do Gene , Marcadores Genéticos , Genética , Predisposição Genética para Doença , Genótipo , Fator de Transcrição MSX1 , Genética , Repetições de Microssatélites , Genética , Polimorfismo Genético
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