RESUMO
Objective To research the effects of progressive time phase selective light (PTPSL)therapy on shift work disorder patients.Methods 24 shift work disorder patients were randomlv divided into PTPSL group and control group.Both groups received treatment for 12 days including 6 days during the night shift time and 6 days during the day shift time continuously.The clinical effects were evaluated by Karolinska Sleepiness Scale (KSS),Leeds sleep evaluation questionnaire (LSEQ) and core body temperature rhythm.Results (1) During the night shift time,PTPSL group patients were better in score LSEQ and KSS(discerned for 39.80 ± 2.89,6.59 ±0.48) than control group' s(discerned for 36.86 ± 3.88,6.98 ± 2.03) ; temperature curve peak phase in PTPSL group moved backward greater than that of control group((8.35 ± 0.82) h vs (7.45 ± 1.05) h) ; and the difference was statistical significance(P < 0.05).During the day shift time,PTPSL group patients were better in score LSEQ and KSS(discerned for 45.57 ± 2.77,6.33 ± 0.48) than control group' s (discerned for 40.35 ± 4.19,6.82 ± 1.03),temperature curve peak phase in PTPSL group moved forward greater than that of control group((7.37 ± 0.94)h vs (6.31 ±1.37)h),and the difference was statistical significance(P<0.05).Conclusion PTPSL can adjust shift sleep disorder patients' mussily sleep rhythm system in order,and make the inner rhythm svstem and external schedules tend to be consistent,and provides a new treatment choice for shift sleep disorders.
RESUMO
Objective:To establish a two-dimensional electrophoresis technology tissue protein of colon carcinnma. Methods: Lysis buffer of different volume was taken to extract tissue proteins of colon carcinoma,and different protein quantities (1mg,2mg,3mg) different preparation ways were taken to establish a two-dimensional electrophoresis. Coomassie brilliant blue was applied to stain protein,and patterns were analyzed. Results:With a molecular mass between 6.5~200ku and isoelectric points (pI) from 3~10,1mg proteins obtained 569 protein spots,2mg 786,and 3mg 835. Pattern of 2mg protein and the sample two were the best. Conclusion:A two-dimensional electrophoresis technology on tissue protein of colon carcinoma has been established successfully.