[ case report of isolated radial nerve palsy physiotherapy]

Isolated radial nerve palsy is caused by pressing this nerve and it is usually caused by the excessive pressure either in the uterus or during the labor. Isolated radial nerve palsy should be considered different from brachial plexus damages. In brachial plexus damages depending on the damage degree and involved spinal roots, different disorders such as disability in brachial abduction, disability in elbow, wrist and digital flexion and extension may be observed, however, in isolated radial nerve palsy weakness is only reported in extension power. In isolated radial nerve palsy depending on the level of pressure and damage, different symptoms are suspected but the most common symptom is inability in extension of wrist and fingers, which is called wrist drop. This phenomenon is a rare condition and usually recuperates by itself. In the present case report, we have described a newborn case with isolated radial nerve palsy. This male newborn was born after a long labor during which his radial nerve was pressed, so he was born with wrist drop. By prescribing Physical therapy modalities including IR [Infrared], Gentle Massage, Electrical Stimulation [IDC], Gentle Rang of Motion, the patient was completely revealed from the disease

[Polyneuropathy in gasoline addiction, a report of three cases]

The polyneuropathy caused by chronic Gasoline Sniffing is reported to be a gradually progressive, symmetric, sensorimotor polyneuropathy. The report of gasoline sniffing by three 12, 16 and18 years old brothers [Respectively 5, 8 and 11 years of gasoline inhalation addiction] that referred with peripheral neuropathy was studied. The conditions were characterized by relatively small reduction in nerve conduction velocity [depending on the severity of nerve involvement] and to some degree in all four limbs with moderate to severe sensory and motor involvement, lower limbs had worse conditions than upper limbs. The neuropathy was attributed to the N-hexane and tetraethyl, the lead components of gasoline. Educational efforts about the dangers of gasoline sniffing are essential

Antimicrobial activity of different lactobacillus species against multi-drug resistant clinical isolates of pseudomonas aeruginosa

Lactobacilli are the well known friendly bacteria for their probiotic activities against pathogens. The inhibitory activity of different strains of lactobacilli either obtained as commercial products or isolated from human feces was investigated against the clinical isolates of Pseudomonas aeruginosa. The isolates were selected as the most resistant strains when challenged with anti-pseudomonal antibiotics already in clinical practice. Both the plate spot test as well as the agar cup method were used for screening of Lactobacillus strains against Pseudomonas aeruginosa. A Lactobacillus acidophilus strain isolated from feces of an Iranian child showed a strong anti-pseudomonal activity [90 percent after 72h incubation] against the multi-drug resistant clinical isolates while a Lactobacillus reuteri strain isolated from a commercial oral product resulted in relatively weak response and a Lactobacillus acidophilus strain isolated from a commercial vaginal product did not show any inhibitory activity. In a kinetic study the lactobacillus sensitive Pseudomonas aeruginosa showed a significant bacteriostatic activity in vitro in the presence of lactobacillus supernatants. Some lactobacilli exhibit significant inhibitory activity against the multidrug resistant clinical isolates of Pseudomonas aeruginosa

Protective effect of pretreatment with thymoquinone against aflatoxin B1 induced liver toxicity in mice

Thymoquinone [TQ] is one of the active components of Nigella sativa. The plant has been used in herbal medicine for treatment of many diseases including liver complications. The present study aimed to investigate protective effects of TQ on Aflatoxin B1 [AFB1] induced liver toxicity in mice. Animals were divided into six groups and treated intraperitoneally. Group 1 [blank] served as vehicle, group 2 [positive control] received AFB1, Group 3 was treated with 9 mg/kg of TQ, Groups 4, 5 and 6 were treated with 4.5, 9 and 18 mg/kg of TQ, respectively. After three consecutive days, except for groups 1 and 3, animals were administered with a single dose of AFB1 [2 mg/kg]. All the animals were killed 24 hrs following the AFB1 administration under ether anesthesia. Biochemical parameters including AST, ALT and ALP in serum samples and glutathione [GSH] and malondialdehyde [MDA] contents in liver homogenates were determined. Liver sections were collected for histopathological examination. Findings of this study showed that AST, ALT, ALP and MDA levels were significantly lower in the TQ treated animals as compared to AFB1 group [group 2]. Furthermore, TQ was able to recover glutathione content [GSH] of liver tissue. The best response, however, was observed with the dose of 9 mg/kg. Liver sections of AFB1 intoxicated mice showed inflammation, necrosis, hyperplasia of kupffer and infiltration of mononuclear cells, dilation of sinusoids and disruption of hepatocytes, while treatment with TQ helped to normalize liver architecture in accordance to biochemical findings. Taken collectively, TQ has a protective role with optimum dose of 9 mg/kg in AFB1 hepatotoxicity

Establishment of a modified in vitro cultivation of protoscoleces to adult echinococcus granulosus; an important way for new investigations on hydatidosis

Echinococcus granulosus, a zoonotic cestode parasite, causative agent of hydatid cyst is endemic in many parts of the world including the Middle East. Study on different aspects of this parasite is very important and valuable. However, working with adult worms which their habitat situated in the small intestine of canids, is dangerous and risky. Achieving such risky situation needs a controlled condition which is cultivation of the organisms in the laboratory. In this regard, cultivation of E. granulosus protoscoleces leading to adult worms was established in the laboratory for the first time in Iran. Under aseptic conditions a number of protoscoleces were cultivated in diphasic S.10E.H medium using CO2 incubator to produce adult worms. Different forms of parasites including pre-segmentation stages [PS1 - PS4] and segmentation stages [S5-S8] and developing stages in segmented worms [S10-S11] were observed and evaluated in these medium. Finally adult worms contained four proglottids with a large and distinct genital pore were observed 50-55 days post cultivation. These parasites do not produce fertile eggs and conclusively do not have risk of hydatid disease transmission to the researchers. The mentioned method for producing E. granulosus adult worms can open a new window for researches and facilitate working on different aspects of hydatidosis especially for diagnosis, protection and treatment studies

new primer pair in ITS1 region for molecular studies on echinococcus granulosus

Echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. The strains characterization seems to be essential for the establishment of a preventive and control strategy in every endemic area. Using DNA based methods for strain /genotype characterizations of E. granulosus have some difficulties, especially access to an efficient and pure concentration of DNA and proper primers. Using grinder method, a pure and high concentration DNA was extracted from 10 human hydatid cysts collected from Isfahan [central Iran] hospitals, and processed for PCR reaction. Using DNASIS, the primers were designed in internal transcribed spacer 1 [ITS1] region, following analysis of 30 E. granulosus nucleotide sequences, extracted from gene bank. This new and specific E. granulosus primer which amplified DNA thoroughly can be applied for molecular studies on echinococcosis