Flow cytometric analysis of peripheral T lymphocyte subpopulations in psoriasis

Psoriasis is a common inflammatory skin disorder, has received attention as a target for new pathogenesis and oriented therapies. Autoimmunity and T lymphocyte subsets are suggested to be involved in the development of psoriasis. The aim of this work is to asses the role of T lymphocyte subsets in the pathogenesis of psoriasis. We investigated the peripheral blood lymphocyte subpopulations obtained from psoriatic patient before and after treatment and from healthy controls, using two colour flow cytometry. We found highly significant suppression of total CD[3+] T Cells and CD[3+] CD[56+] NKT lymphocytes in psoriatic patient as compared to control. Also, we observed significant reduction of T helper cells in patients as compared to control. The highly significant reduction of CD[3+] T cell and CD[3+] CD[56+] NKT lymphocytes proved their actively involvement in the development of psoriasis

Serum level of IL13 and expression of BCL2 in Behcet's disease

BCL2 family is a large family of apoptosis regulating proteins consisting of both blockers and promoters of cell death. Immunological processes and a variety of cytokines may play a role in pathophysiological process. Defective regulation of programmed cell death [apoptosis] also play a role in development of Behcet's disease. To investigate the level of BCL2 and IL13in BD and to determine their to relation monitory disease activity. This study was conducted on thirty patients [15 active and 15 inactive] and 15-health control, the activity of BD was evaluated according to international study group for BD disease, using ELISA technique for IL13 and flow cytometry for BCL2. Elevated serum levels of IL13 in patient with active BD than inactive and both had elevated levels than control[P< 0.01] and also the serum levels of Bcl2 was elevated in patient with active BD than inactive and control[P< 0.01]. The data suggested that IL13 and BCL2 could be involved in the pathogenesis of BD and its serum levels can be used as marker to monitor disease activity