RESUMO
OBJECTIVE: To establish a method for detecting 41 mineral elements such as beryllium, magnesium and calcium in whole blood by inductively coupled plasma-mass spectrometry(ICP-MS). METHODS: After the whole blood samples were diluted 1∶20 in a mixed solution containing 0.10% Triton X-100 and 0.50% nitric acid, 41 mineral elements in whole blood samples such as beryllium, magnesium and calcium were determined by the ICP-MS, using scandium, rhodium and iridium as the internal standard elements. RESULTS: The linear correlation coefficient was >0.999 0 in these 41 elements such as beryllium, magnesium and calcium in the whole blood. The detection limit was 0.006-247.000 μg/L, the quantification lower limit was 0.020-822.000 μg/L. The recovery rate of standard addition was 84.00%-109.40%. The within-run and between-run relative standard deviation were 0.66%-7.34% and 0.56%-8.22%, respectively. The samples could be stored at-20 ℃ for at least 14 days. CONCLUSION: This method is simple, rapid and sensitive, with good precision and reliable results. It can be used for detecting multiple mineral elements in whole blood of normal and occupational population.
RESUMO
In this study, the distribution of five Alzheimer's disease (AD)-related single nucleotide polymorphisms (SNPs) in the Han population was examined in combination with the evaluation of clinical cognition and brain pathological analysis. The associations among SNPs, clinical daily cognitive states, and postmortem neuropathological changes were analyzed in 110 human brains from the Chinese Academy of Medical Sciences/Peking Union Medical College (CAMS/PUMC) Human Brain Bank. APOE ε4 (OR = 4.482, P = 0.004), the RS2305421 GG genotype (adjusted OR = 4.397, P = 0.015), and the RS10498633 GT genotype (adjusted OR = 2.375, P = 0.028) were associated with a higher score on the ABC (Aβ plaque score, Braak NFT stage, and CERAD neuritic plaque score) dementia scale. These results advance our understanding of the pathogenesis of AD, the relationship between pathological diagnosis and clinical diagnosis, and the SNPs in the Han population for future research.
Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteína ADAM10 , Genética , Doença de Alzheimer , Genética , Patologia , Secretases da Proteína Precursora do Amiloide , Genética , Antiporters , Genética , Apolipoproteína E4 , Genética , Povo Asiático , Genética , Encéfalo , Patologia , Disfunção Cognitiva , Genética , Patologia , Predisposição Genética para Doença , Proteínas de Membrana , Genética , Polimorfismo de Nucleotídeo ÚnicoRESUMO
OBJECTIVE: To establish a method for detecting the contents of 12 toxic elements such as beryllium,vanadium and thallium in human whole blood,urine and hair by inductively coupled plasma-mass spectrometry( ICP-MS).METHODS: Whole blood( 0.25 mL) and urine( 10.00 mL) were diluted 20 times into suspension by 0.1% TritonX-100 + 0.5% nitric acid,and analyzed by ICP-MS.The hair( 0.20 g) was mixed with nitric acid and digested by microwave,then made up to 10.00 mL with purified water.The analysis was performed by ICP-MS.RESULTS: The linear correlation coefficients of all the 12 elements,such as beryllium,vanadium,chromium,manganese,cobalt,nickel,arsenic,cadmium,tin,antimony,tellurium and lead were≥0.999 5.The detection limit for whole blood and urine was 0.097-1.995 μg/L,and the detection limit for hair was 0.001-0.012 μg/g.The recovery rates for whole blood,urine and hair were 92.3%-105.0%,93.7%-115.5% and 92.5%-111.0%,respectively.The within-run relative standard deviation( RSD) were 0.7%-5.8%,0.8%-4.6% and 2.2%-8.4%,respectively; the between-run RSD were 1.6%-7.1%,3.5%-7.5% and 2.8%-8.8%,respectively.CONCLUSION: This method has good accuracy,high sensitivity and good precision,which is suitable for rapid screening for various elements in human biological samples.
RESUMO
Objective To study the clinical features of aged patients with sudden cardiac death(SCD) for improving the resuscitation rate. Methods Hie usual etiology of SCD,electrocardiogram(ECG),echocardiography, dynamic electrocardiography(DCG), X-ray and electrolytes were analyzed. Results Most sudden death had inducement. Electrolytes disorder,ventricular ectopic beats,high grade atrioventricular block, auxocardia, left ventricular hypertrophy, cardiac insufficiency and myocardial ischemia could cause serious arrhythmias (.all P <0.05). Conclusion It could be related to heart functional class,left ventricular enlargement,myocardial ischemia,hypokalemia in aged patients with SCD.