experimental study of the effect of cyclosporine: a on normal versus inflamed gingiva

The present study was designed to investigate the histological feature inducedby cyclosporin-A [Cs-A] on clinically healthy and induced inflammatorycondition of the gingiva. Twenty four albino rats were used. Silk ligaturewas tighted around the mandibular incisors to act as a local irritating factorin 12 of them. Rats subdivided into two equal groups. Control group injectedwith 30 mg/Kg/body weight of cyclosporin A and included 6 rats with clinicallyhealthy gingiva and other 6 of induced inflamed gingival tissues. The resultsdemonstrated a highly hyperplastic feature in the epithelium associated withincreased collagen deposition with few fibroblasts, inflammatory cellinfiltrates in the underlying connective tissue. No significant difference ofgingival tissue changes or level of protein synthesis were detected betweenclinically healthy and inflamed gingival tissues upon Cs-A medication. It wasconcluded that, Cs-A induced gingival enlargement, and this work provides ananimal model for further investigation of other factors that might contributewith Cs-A in production of gingival over growth

Clinical and bacteriological evaluation of the periodontal therapy by the placement of acrylic strips containing antibacterial drugs into the periodontal pockets

The role of specific Gram negative bacteria in the etiology and pathogenesis of periodontal disease has been markedly reported in the last two decades. This study evaluates the effect of local application of tetracycline or metronidazole on the subgingival bacterial as well as the clinical condition of the gingiva. Twenty four periodontitis patients were included in this study, each patient had either metronidazole or tetracycline, incorporated into acrylic strips inserted subgingivally once weekly for 3 weeks. Subgingival plaque samples were obtained from each patient immediately prior to insertion and after the removal of the strips. The collected bacterial plaque samples were cultured and identified. The results showed an improved gingival status and pocketing. Bacteriologically, the two drugs peoduced an increased number of cocci species with marked reduction of Gram negative rods, however metronidazole was more effective than tetracycline

Role of T and B lymphocytes in spontaneous lymphocyte proliferation in chronic inflammatory periodontal disease

Spontaneous lymphocyte responses of whole mononuclear cells [MNCs, T-lymphocytes and B-lymphocyte in patients with periodontal disease and theircontrols were studied in this work utilizing the tritiated thymidine uptakeassay. The periodontitis patients exhibited lower lymphoproliferativeresponse of MNCs compared with the controls. However, theB-lymphoproliferative response of patients showed the highest proliferativeresponse compared with that of all cell types studied. This finding maysuggest that the elimination of T-cell affects the hyperresponsiveness ofB-lymphocyte state