Rapid detection of the genotyping of hepatitis C virus using DNA chip with coloration methods / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To develop a new DNA chip with coloration, which can be used for rapid and economical detection of the genotyping of hepatitis C virus (HCV).</p><p><b>METHODS</b>Probes and primers were designed according to the sequence of HCV 5' non-coding region (5' NCR) to fabricate DNA chip. Experimental group consisted of 60 positive serum samples and control group consisted of 20 negative serum samples. To obtain the aimed gene, then they were hybridized with DNA chip. Finally, the results showed in a nylon film. The results of DNA sequencing of samples were used as the control in double blind experimental.</p><p><b>RESULTS</b>Using DNA chip, HCV was detected in positive of all serum specimens of experimental group and negative in control group. The determination of HCV genotype by DNA chip showed corresponding rate of 96.7% with those by sequence assay.</p><p><b>CONCLUSION</b>It showed higher specialty and sensitivity using DNA chip to detect the genotype of HCV. It would be valuable for the clinical genotyping of HCV</p>

Establishing DNA chip technique for detecting hepatitis C virus genotypes and primary application / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To establish a new method based DNA chip technique for detecting HCV genotypes.</p><p><b>METHODS</b>Genotyping probes were designed according to the sequence of HCV 5' NCR to generate DNA chip. The probes on DNA chip contains 5 major genotypes and 8 subtypes. The DNA fragment amplified by labeling Cy5 fluorescence was hybridized with DNA chip.</p><p><b>RESULTS</b>Fifty-five out of 65 isolates detected by DNA chip belonged to 1b- DNA sequencing of form a part of the isolates was used as the control. The results of both were completely consistent.</p><p><b>CONCLUSION</b>The method is simple and rapid with high specificity and sensitivity. It can be applied in detection of HCV RNA and genotypes.</p>