RESUMO
Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to Bax ratio was also seen in myocytes treated with H2S after H2O2-induced stress. Our findings indicated a therapeutic potential for H2S in preventing myocyte death following ischemia/reperfusion.
Assuntos
Animais , Ratos , Apoptose/efeitos dos fármacos , Mioblastos Cardíacos/efeitos dos fármacos , Peróxido de Hidrogênio , Antioxidantes/farmacologia , Valores de Referência , Sulfetos/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Western Blotting , Reprodutibilidade dos Testes , Espécies Reativas de Oxigênio/metabolismo , Apoptose/fisiologia , Estresse Oxidativo/efeitos dos fármacos , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Mioblastos Cardíacos/metabolismo , Potencial da Membrana Mitocondrial , Citometria de Fluxo/métodos , Sulfeto de Hidrogênio/farmacologiaRESUMO
<p><b>OBJECTIVE</b>To ensure and enhance the quality of multi-central large sample clinical trials of acupunc ture and moxibustion.</p><p><b>METHODS</b>Take the multi-central clinical trials supported by National Key Technology R &- D Program of "the 11th Five-year Plan" as an example to introduce the duty of monitors and simply describe the formulation of the monitoring plan, the monitoring procedure and contents of the monitoring plan and treatment of the commonly-seen problems.</p><p><b>CONCLUSION</b>Quality monitoring work in multi-central large sample clinical trials of acupuncture and moxibustion is equally serious and scientific with clinical medical trials and it is an important way to guarantee the credible results.</p>