Genetic fingerprints of some bacillus spp. isolates gathered from different locations at Saudi Arabia

Twelve Bacillus spp. isolates gathered from different locations at Saudi Arabia were fingerprinted by random amplified polymorphic DNA [RAPD] markers. The results revealed high levels of polymorphism among the studied isolates. Of many 10-mer random primers screened, five gave distinct and reproducible polymorphic DNA amplification patterns. The primers are A3, B6, B10, B11 and C12. The number of the generated bands was primer and genotype dependent. A total of 36 amplified polymorphic DNA bands were generated across the studied isolates with 9 bands being unique. All primers gave a number of unique bands that used for discrimination of some isolates. No primer was able to generate a unique banding pattern for all the studied isolates. However, combinations of all data derived from the RAPD profiles generated by the five utilized primers produced a unique fingerprint for each isolate. Similarity coefficients among the studied isolates were estimated after pooling all the gained data. The levels of similarity between the studied isolates showed low values. It reached the highest value of 0.938 between the two isolates N2 and M2, while the minimum value was 0.118 recorded between the two isolates M1 and W1. The generated dendrogram clustered the three isolates E1, N2 and M2 together and located W1 in a separate cluster

Genetic fingerprinting and relationships of some corynebacterium isolates based on DNA polymorphism

Genetic differences and relationships among six Corynebacterium isolates of different geographic origin from Saudi Arabia were studied based on RAPD-PCR polymorphism. In this analysis many random 10-mer primers were screened, but only five primers [A3, A11, B10, B11 and Z6] were able to generate repeatable and informative products among the DNA samples of the studied bacterial isolates. A total of 47 polymorphic bands were generated by the five primers [100% polymorphism]. Of these bands 23 were scored as unique polymorphic bands. The two primers A11 and B10 were found to be the most effective ones in generating unique bands. The former primer generated 8 unique bands in six isolates while the latter primer produced six unique bands in four isolates. In addition, the most pronounced non unique bands categorized the studied isolates into many class patterns. Combination of all data provides a considerable potentiality for discriminating each Corynebacterium isolate by one or more unique bands or a group of combined class patterns. RAPD data were combined together and used to estimate similarity coefficients and to construct a dendrogram that estimates the relationships among the studied Corynebacterium isolates. The similarity coefficient values varied from zero to 0.44% indicating considerable variability within the studied isolates Based on this dendrogram the studied isolates were separated into two main groups. Such clustering in most cases was well correlated to the geographic collection sites