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1.
Korean Journal of Veterinary Research ; : 197-201, 2014.
Artigo em Coreano | WPRIM | ID: wpr-68071

RESUMO

In Korea, brucellosis has been reported periodically in cattle and rarely in dogs; however, it has not previously been screened in domestic animals such as elk, pigs and goats. To investigate the serological prevalence, serum samples were taken from the aforementioned animals annually during 2007-2013 and screened by the rose-bengal test (RBT) or modified RBT, after which positive sera were evaluated by the standard tube agglutination test (STAT). Finally, RBT and STAT-positive sera were confirmed by competitive-ELISA. Brucella abortus biovar 1 was isolated from three elk that were shown to be positive serologically in 2008. There was no evidence of brucellosis in pigs. Based on serological monitoring and investigation of etiological agents, there is no evidence of outbreak of brucellosis in elk, pigs or goats of Korea since 2008. However, the possibility for brucellosis from cattle to affect these other livestock exists; therefore, extensive and continuous serological monitoring is required to maintain their brucellosis-free status.


Assuntos
Animais , Bovinos , Cães , Testes de Aglutinação , Animais Domésticos , Brucella abortus , Brucelose , Cabras , Coreia (Geográfico) , Gado , Prevalência , Testes Sorológicos , Suínos
2.
Korean Journal of Veterinary Research ; : 147-150, 2014.
Artigo em Coreano | WPRIM | ID: wpr-129079

RESUMO

Q-fever is a vector-borne (Coxiella [C.] burnetii) zoonotic disease that is an increasing public health concern. To date, some research about Q-fever prevalence in dairy herds and human patients has been reported in Korea, but information about Korean native cattle is scarce. To measure the prevalence rates of C. burnetii in Korean native cattle, a total of 1,095 bovine serum samples collected during 2010~2013 were analyzed with an enzyme-linked immunosorbent assay. Sixty-eight heads of cattle were diagnosed as positive and while 19 heads were suspected (positive rate = 6.2%). Interestingly, Jeju province had a seropositivity rate six times greater than that of other provinces (18.9% vs. 3.2%). High seroprevalence might be caused by wide distribution of ticks in Jeju province compared to other regions. Based on these data, extensive monitoring of C. burnetii infection in cattle, tick distribution, and climate changes is required.


Assuntos
Animais , Bovinos , Humanos , Mudança Climática , Ensaio de Imunoadsorção Enzimática , Cabeça , Coreia (Geográfico) , Prevalência , Saúde Pública , Estudos Soroepidemiológicos , Carrapatos , Zoonoses
3.
Korean Journal of Veterinary Research ; : 147-150, 2014.
Artigo em Coreano | WPRIM | ID: wpr-129065

RESUMO

Q-fever is a vector-borne (Coxiella [C.] burnetii) zoonotic disease that is an increasing public health concern. To date, some research about Q-fever prevalence in dairy herds and human patients has been reported in Korea, but information about Korean native cattle is scarce. To measure the prevalence rates of C. burnetii in Korean native cattle, a total of 1,095 bovine serum samples collected during 2010~2013 were analyzed with an enzyme-linked immunosorbent assay. Sixty-eight heads of cattle were diagnosed as positive and while 19 heads were suspected (positive rate = 6.2%). Interestingly, Jeju province had a seropositivity rate six times greater than that of other provinces (18.9% vs. 3.2%). High seroprevalence might be caused by wide distribution of ticks in Jeju province compared to other regions. Based on these data, extensive monitoring of C. burnetii infection in cattle, tick distribution, and climate changes is required.


Assuntos
Animais , Bovinos , Humanos , Mudança Climática , Ensaio de Imunoadsorção Enzimática , Cabeça , Coreia (Geográfico) , Prevalência , Saúde Pública , Estudos Soroepidemiológicos , Carrapatos , Zoonoses
4.
Korean Journal of Veterinary Research ; : 25-31, 2012.
Artigo em Coreano | WPRIM | ID: wpr-145340

RESUMO

Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.


Assuntos
Humanos , Feto Abortado , Brucella , Brucella canis , Brucelose , Células Clonais , Ensaio de Imunoadsorção Enzimática , Animais de Estimação , Placenta , Proteínas Ribossômicas , Sensibilidade e Especificidade , Médicos Veterinários
5.
Korean Journal of Veterinary Research ; : 151-158, 2011.
Artigo em Coreano | WPRIM | ID: wpr-159633

RESUMO

The study evaluated whether a transgenic carrot vaccine could induce a K88-specific immune response in sows and whether the resultant maternal antibody could protect piglets against enterotoxigenic Escherichia coli (ETEC) K88ac infection. Sows (n = 15) selected randomly from a farm in Korea were assigned to three groups (n = 5 per group: control [untreated]), group A (orally inoculated with a non-transgenic and transgenic carrot vaccines at 2 and 4 weeks ante partum, respectively), and group B (conventionally vaccinated according to the manufacturer's instructions). After 7 days of lactation, 5 piglets selected randomly from each group were challenged with 1 x 1010 colony forming units/mL ETEC K88ac. Group C had the lowest mean fecal consistency score on post-challenge days 1 and 7. Histiologically, On post-challenge day 7, group C showed an increased duodenum and ileum villus:crypt ratio, compared to group A in the duodenum, with group B displaying the highest ratio. Groups B and C had more increased villus width than group A in the jejunum. Group C displayed the greatest increase in villus width in the ileum. The colostrums and serum from groups B and C displayed higher concentrations of IgA and IgG against ETEC K88, compared to group A. Based on the results, it was concluded that the transgenic carrot vaccine in sow per oral may have an effect on preventing piglet diarrhea as good as commercial recombinant vaccine.


Assuntos
Feminino , Formigas , Colostro , Daucus carota , Diarreia , Duodeno , Escherichia coli Enterotoxigênica , Íleo , Imunoglobulina A , Imunoglobulina G , Jejuno , Coreia (Geográfico) , Lactação , Vacinas
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