Study of antimutagenic and mutagenic effect of different chemicals on clinically isolated strains of pseudomonas aeruginosa

This project was undertaken to study the effect of twelve different compounds to test their antimutagenic and ii mutagenic activity against clinically isolated strains of Pseudomonas aeruginosa. The effect of these compound was estimated by counting the number of rifampicin resistant colonies growing in a particular time in a it compound. The results were interpreted by plotting graphs between lOg N/No [Rif R Colonies/ml] and time to estimate the forward mutation rate. The results revealed that Acridine, Basic fuchsin, Caffeine, Cyclohexamide, Zehidium bromide and Histidine probably have an antimutagenic effect, while [steine, Folic acid, Ethyl-methane. ulphonate, Manganous Chloride and N-nitrosodietylamine acted as mutagen. E.coli was used as control through out the study

Genetic study of anti-microbial drug resistance strains of pseudomonas aeruginosa

The present work was carried out to study the genetic factors for multi-drug resistance in clinically isolated strains of Ps. aeruginosa. Conjugal transfer of resistance factor between Ps. aeruginosa strains was observed, while no transfer was found from Ps. aeruginosa to E. coli strains. The DNA analysis by gel-ectrophoresis revealed the presence of plasmid in one strain [AQ 003] only. As no conjugal transfer or presence of plasmid was observed in other strains, it is suggested that in these strains resistance may be of chromosomal or genetic origin

Mutability in clinically cultured strains of Escherichia coli

The present work was undertaken to study the mutability in clinically cultured strains of Escherichia coil [E. coli]. The mutability studies were carried out by using two mutagens i.e. ultraviolet light and Ethyl Metnane Sulphonate [EMS]. The studies reveal that these clinically isolated strains do not differ significantly from other standard laboratory strains of E. coil. The mutability was enhanced from 10[-4] to 10[-2] by use of mutagens

Hepatitis B virus surface antigenemia in voluntary blood donors and patients with acute hepatitis

Using the agglutination technique for the detection of virus hepatitis B surface antigen [HBs Ag], blood samples from 1877 apparently healthy subjects [1258 males, 619 females] were tested. Of these, 1258 cases [807 males, 451 females] were college students and 619 subjects [592 males, 27 females], were from the mixed community. All of them came to donate blood at the time of Ojri Camp disaster. The over-all positivity rate for college students was 1.8% [males: 1.98%, females: 1.55%] and 2.9% for mixed community [males: 3.04%, females: 0%]. In contrast, the positivity rate in 166 cases of acute hepatitis patients was much higher i.e. 24.7% [males: 21.8%, females: 31.9%]. More work is required to study the epidemiology of HB virus infection in Pakistan. The cost effectiveness of routine screening of blood donors needs further evaluation