Prevalence of high-risk human papillomavirus by cobas 4800 HPV test in urban Peru

Background: Molecular tests allow the detection of high-risk human papillomavirus in cervical samples, playing an important role in the prevention of cervical cancer. Objectives: We performed a study to determine the prevalence of HPV 16, HPV 18 and other high-risk human papillomavirus (pool 12 genotypes) in Peruvian females from diverse urban areas using the cobas 4800 HPV test. Methods: Routine cervical samples collected in our laboratory were analyzed by cobas 4800 HPV test. Results: A total of 2247 samples from female patients aged 17–79 years were tested. high-risk human papillomavirus was positive in 775 (34.49%) samples. Of these, 641 (82.71%) were single infections and 134 (17.29%) were multiple infections. The positivity rates for HPV 16, HPV 18, and other high-risk human papillomavirus were 10.77%, 2.0%, and 28.08%, respectively. In multiple high-risk human papillomavirus infections, the concomitance of HPV 16 and other high-risk human papillomavirus was more prevalent (13.42%). Conclusion: Our study showed high prevalence of high-risk human papillomavirus in urban Peru, mainly among young women. In both single and multiple infections other high-risk human papillomavirus were more prevalent than HPV 16 and HPV 18, which might influence vaccine impact in our country. Furthermore, the cobas 4800 HPV test may be considered a useful tool for HPV molecular diagnosis. .

Prevalencia del virus del papiloma humano de alto riesgo en el Perú / Prevalence of human papilloma virus high-risk in Peru

Introducción: El virus del papiloma humano de alto riesgo (VPH-AR) juega un rol importante en la génesis del cáncer cervical. En nuestro país son pocos los estudios con respecto a la epidemiología de VPH. Objetivo: En el presente trabajo estudiamos la prevalencia total de VPH-AR, así como la frecuencia de VPH 16, VPH 18 y otros VPH-AR (pool de 12 genotipos) en zonas urbanas del Perú usando el cobas 4800 HPV Test. Material y métodos: Se análizaron 2247 muestras cervicales mediante el kit cobas 4800 HPV Tst. El rango de edad de las pacientes fue de 17 a 79 años. Resultados: Se determinó una prevalencia total de VPH-AR de 34.49% (775/2247). Tanto en infecciones simples y múltiples, los otros VPH-AR fueron más comunes que el VPH 16 y el VPH 18. Conclusiones: Nuestro estudio mostró una alta prevalencia de VPH-AR, principalmente en mujeres jóvenes. En América Latina nos encontramos como uno de los países con mayor prevalencia de VPH.

Introduction: The high-risk human papillomavirus (HR-HPV) plays an important role in the genesis of cervical cancer. In our country there are few studies regarding the epidemiology of HPV. Objective: In this paper we perform a study to determine the overall prevalence of HR-HPV, and the frequency of HPV 16, HPV 18 and other HR.HPV (pool 12 genotypes) in urban areas of Peru using the cobas 4800 HPV Test. Material and methods: 2247 cervical samples were analyzed by the cobas 4800 hpv Test. The patients were from 17 to 79 years old. Results: The overall prevalence of HR-HPV was 34.49% (775/2247). Both single and multiple infections, other HR-HPV were more common than HPV 16 and HPV 18. Conclusions: Our study showed a high prevalence of HR-HPV, mainly in young women. In Latin America we are as one of the countries with the highest prevalence of HPV.