RESUMO
Our aim was to apply DNA chip technology as a diagnostic tool in infertility research and clinics. Six loci, including a sex-determining region on the Y chromosome and five sequence-tagged sites in azoospermia-factor regions were investigated in infertile male patients. Our method produced a sensitive signal, which showed the presence or absence of the STS regions on the Y chromosome. The results from 93 patients with non- obstructive azoospermia, oligoathenoteratozoospermia, or oligozoospermia were identical when analyzed with either the DNA chip technique or conventional PCR-gel electrophoresis. We have demonstrated its application in the molecular diagnosis of male infertility. This system provides an economic and high-throughput method for detecting the deletion of genomic DNA sequences of large groups of infertile patients, and a completely new approach to male infertility screening. The application of DNA chip technology to identify Yq deletions can also facilitate our understanding of male infertility.
Assuntos
Feminino , Humanos , Masculino , Deleção Cromossômica , Cromossomos Humanos Y/genética , Análise Mutacional de DNA/métodos , Eletroforese em Gel de Ágar , Infertilidade Masculina/diagnóstico , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Reação em Cadeia da Polimerase , Valor Preditivo dos Testes , Proteínas de Plasma Seminal/genética , Sensibilidade e Especificidade , Sitios de Sequências Rotuladas , Aberrações dos Cromossomos SexuaisRESUMO
A 26-year-old-male with a foreign body stone of the bladder caused by gold thread acupuncture, which was inserted 10 years ago for the treatment of lumbago and migrated spontaneously from the right renal area to the bladder through upper urinary tract, was presented. He was managed by extraction of foreign body stone with foreign body forceps and endoscopic manipulation. Authors report this unusual case with review of some literatures.
Assuntos
Acupuntura , Corpos Estranhos , Dor Lombar , Instrumentos Cirúrgicos , Cálculos da Bexiga Urinária , Bexiga Urinária , Sistema UrinárioRESUMO
A 26-year-old-male with a foreign body stone of the bladder caused by gold thread acupuncture, which was inserted 10 years ago for the treatment of lumbago and migrated spontaneously from the right renal area to the bladder through upper urinary tract, was presented. He was managed by extraction of foreign body stone with foreign body forceps and endoscopic manipulation. Authors report this unusual case with review of some literatures.
Assuntos
Acupuntura , Corpos Estranhos , Dor Lombar , Instrumentos Cirúrgicos , Cálculos da Bexiga Urinária , Bexiga Urinária , Sistema UrinárioRESUMO
Rhadomyosarcoma of prostate is relatively rare, malignant tumor in urologic field. This tumor is believed to arise from primitive mesenchymal cells and the growth usually protrudes into the bladder and urethra causing obstruction with urinary retention. The excretory urogram and cystourethrogram demonstrate the displacement of the bladder and elongation of posterior urethra. Current treatment of this tumor consists of surgery, irradiation, and combination chemotherapy. Despite above mentioned treatment, the prognosis of prostatic rhabdomyosarcoma is usually poor. We are presenting a case of embryonic rhabdomyosarcoma in 15-year old boy with the review of articles.