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1.
Indian J Exp Biol ; 2003 Dec; 41(12): 1476-8
Artigo em Inglês | IMSEAR | ID: sea-60036

RESUMO

Influence of different environmental factors on accumulation of wedelolactone, a potential anti-hepatotoxic principle of E. alba in shoot cultures was investigated. A significant increase in the content of wedelolactone due to kinetin treatment, temperature change and photoperiod alteration in shoot cultures was observed. Incorporation of phenylalanine in the medium also increased content of wedelolactone significantly in a dose-dependent manner.


Assuntos
Cromatografia em Camada Fina , Cumarínicos/metabolismo , Eclipta/metabolismo , Concentração de Íons de Hidrogênio , Brotos de Planta/metabolismo
2.
Indian J Exp Biol ; 2002 Dec; 40(12): 1382-6
Artigo em Inglês | IMSEAR | ID: sea-59400

RESUMO

Adventitious roots, generated using leaf explants of P. nodiflora, and meristem explants of L. reticulata, were cultured on Murashige and Skoog (MS) medium supplemented with napthylacetic acid (2 microM) and indole butyric acid (3 microM) respectively. After 30 days, subculturing of roots in liquid MS medium with napthylacetic acid (1.5 microM) for P. nodiflora and indole butyric acid (3 microM) for L. reticulata afforded considerable increase in root mass. HPTLC profiles and microscopic examination of transverse sections of in vitro and naturally grown roots provided information on secondary metabolite accumulation vis-à-vis developmental stages of the root.


Assuntos
Animais , Apocynaceae/fisiologia , Cromatografia em Camada Fina , Raízes de Plantas/crescimento & desenvolvimento , Regeneração , Verbenaceae/fisiologia
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