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Indian J Exp Biol ; 2010 Jan; 48(1): 17-25
Artigo em Inglês | IMSEAR | ID: sea-144091

RESUMO

Meshed pigmented iris epithelium along with neural retina of tadpoles of the frog Euphlyctis cyanophlyctis were found to undergo dedifferentiation and subsequently transdifferentiate into lens in culture medium. During lag period, depigmentation (dedifferentiation) occurred in many cells. When culture became confluent 3-4 weeks after seeding tiny lens like structures differentiated from foci of cultured pigmented iris epithelium cells. The percentage of lens formation was higher in vitamin A treated cases. The culture system appears to be a suitable for investigating the changes occurred during trans-differentiation of pigmented epithelial cells into lens.


Assuntos
Animais , Transdiferenciação Celular/efeitos dos fármacos , Iris/citologia , Iris/efeitos dos fármacos , Larva/citologia , Larva/crescimento & desenvolvimento , Cristalino/citologia , Cristalino/efeitos dos fármacos , Cristalino/crescimento & desenvolvimento , Epitélio Pigmentado Ocular/citologia , Epitélio Pigmentado Ocular/efeitos dos fármacos , Ranidae/anatomia & histologia , Ranidae/crescimento & desenvolvimento , Técnicas de Cultura de Tecidos , Vitamina A/farmacologia
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