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Chinese Journal of Cardiology ; (12): 661-665, 2007.
Artigo em Chinês | WPRIM | ID: wpr-307224

RESUMO

<p><b>OBJECTIVE</b>To study the effect of peroxisome proliferators activated receptors (PPAR) alpha, gamma ligand on ATP-binding cassette transporter A1 (ABCA1) and caveolin-1 expressions and cholesterol, ox-LDL contents in human monocyte derived foam cells.</p><p><b>METHOD</b>Malondialdehyde (MDA) was measured by TBARS method, ox-LDL detected by ELISA method, cholesterol measured by fluorescence spectrophotometric method, ABCA1, caveolin-1 mRNA and protein expressions determined by RT-PCR and Western blot, in human monocytes, foam cells [human monocyte-derived macrophage induced by myristate acetate (PMA) further treated with 50 mg/L ox-LDL for 24 h], foam cells plus 10 micromol/L pioglitazone for 48 h, foam cells plus 5 micromol/L clofibrate for 48 h.</p><p><b>RESULT</b>The intracellular total cholesterol (TC), free cholesterol (FC), cholesteryl ester (CE), ox-LDL and lipid peroxide were significantly increased and the membrane expressions of ABCA1, caveolin-1 were down-regulated in foam cells compared to monocytes (all P < 0.05) and these changes were significantly attenuated by cotreatment with PPARalpha, gamma ligand.</p><p><b>CONCLUSION</b>The anti-atherosclerosis effects of PPARalpha, gamma ligand are related to reducing cholesterol contents and up-regulating ABCA1, caveolin-1 expressions in foam cells.</p>


Assuntos
Humanos , Transportador 1 de Cassete de Ligação de ATP , Transportadores de Cassetes de Ligação de ATP , Metabolismo , Caveolina 1 , Metabolismo , Linhagem Celular , Colesterol , Genética , Metabolismo , Células Espumosas , Metabolismo , Expressão Gênica , Malondialdeído , Metabolismo , Monócitos , Metabolismo , PPAR alfa , Metabolismo , PPAR gama , Metabolismo
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