Ultrastructure and TUNEL staining on inhibition of Rubus alceaefolius total alkaloids for apoptosis of liver in rat models of acute hepatitis / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the anti-apoptosis effects of Rubus alceaefolius total alkaloids in rats with hepatic injury.</p><p><b>METHOD</b>The hepatic injury model of rat was induced by intraperitoneal injection with CCl4. Sixty SD rats were randomly divided into the normal group, the model group, the R. alceaefolius total alkaloids intervened group, and the bifendate intervened group. The expressions of the levels of liver cell apoptosis were determined by TUNEL. Ultrastructures of the liver cells were observed with transmission electron microscope.</p><p><b>RESULT</b>Compared with the model group, the degree of hepatic injury and the positive expressions of apoptosis in liver tissues in the R. alceaefolius total alkaloids intervened groups and the bifendate intervened group were significantly lower.</p><p><b>CONCLUSION</b>R. alceaefolius total alkaloids could reduce the pathological changes and degree of hepatic injury in rats, which may be partially through inhibiting the expressions of apoptosis in liver tissue.</p>

Optimization of the Extraction Process of Qianliening Granules by Orthogonal Test / 浙江中医药大学学报

[Objective]To filter and optimize extractive technology of Qianliening granules.[Methods]The optimization extractive technology of Qianliening granules was investigated using orthogonal design with the availability component extracting from the drug as the index.[Results]The optimal condition for the extraction of Radix Astragali group was 10 folds amount of water,3 times,1.5 hours each time.The optimal condition for the extraction of Rheum officinal Baill.group was 6 folds amount of 60% alcohol,2 times,two hours and 1.5 hours each time.[Conclusion]The optimized extractive technology is scientific and efficient.