Diterpenoid alkaloids in aerial parts of Aconitum transsectum / 中草药

Objective: To study the diterpenoid alkaloids in the aerial parts of Aconitum transsectum. Methods The compounds were isolated and purified by silica gel and Sephadex LH-20 chromatography from the aerial parts of A. transsectum, and the structures were identified by spectral analysis (1H-NMR, 13C-NMR, MS). Results: A total of 16 compounds were isolated from A. transsectum and characterized as longtoaconitine A (1), 14-acethyltalatisamine (2), 8-O-methylsachaconitine (3), talatisamine (4), vilmorrianine D (5), 14-acetylsachaconitine (6), forestine (7), chasmanine (8), crassicausine (9), homochasmanine (10), crassicautine (11), 8-deacetylyunaconitine (12), liljestrandisine (13), vilmorrianine B (14), yunaconitine (15), and isotalatizidine (16). Conclusion: Compounds 1-16 are isolated from aerial parts of A. transsectum for the first time, and compounds 1, 3, 9-11, 13-14, and 16 are isolated from A. transsectum for the first time.

Expression of miR-1285 in ovarian cancer tissues and its influence on proliferation and apoptosis of cancer cells / 重庆医学

Objective To explore the expression of microRNA-1285 (miR-1285) in ovarian cancer tissues,and to analyse the effects of miR-1285 expression on proliferation and apoptosis of human ovarian cancer cell lines in vitro.Methods The expressions of miR-1285 in cancer tissues and corresponding adjacent tissues from patients with ovarian cancer were detected by using fluorescence quantitative PCR.At the same time,human ovarian cancer cell lines,including SKOV3 and OVCAR3,were transfected with miR-1285 mimics in vitro,the proliferation activity of cells successfully transfected with miR-1285 was detected by MTT assay,and the apoptotic cells were detected by flow cytometry.Results The results of fluorescence quantitative PCR showed that,for patients with ovarian cancer,the expression level of miR-1285 in ovarian cancer tissues was lower than that in the corresponding adjacent tissues,the difference was statistically significant (P＜0.05).The results of experiment in vitro showed that compared with the control group,the proliferation activity of SKOV3 cells transfected with miR-1285 mimics was decreased,and the rate of apoptosis was significantly increased,there were statistically significant differences (P＜0.05);similarly,compared with the control group,the proliferation activity of OVCAR3 cells transfected with miR-1285 mimics was decreased,and the rate of apoptosis was significantly increased,there were statistically significant differences (P＜0.05).Conclusion The expression level of miR-1285 in tumor tissues of patients with ovarian cancer is decreased,which indicates that miR-1285 may be involved in the occurance and progression of ovarian cancer;the experiment in vitro shows that miR-1285 may be closely related to the proliferation and apoptosis of ovarian cancer cells.

Effect of paclitaxel combined with lobaplatin in the treatment of 58 patients with LACC / 重庆医学

Objective To investigate the effect of paclitaxel combined with platinum on neoadjuvant chemotherapy in patients with locally advanced cervical cancer (LACC).Methods A total of 58 cases with LACC were treated with neoadjuvant chemotherapy in our hospital were selected,of which 29 patients (group A) were treated with paclitaxel plus lobaplatin and 29 patients (group B) were treated with paclitaxel plus cisplatin.Both groups received Radical cervical cancer surgery.The short-term efficacy,serum tumor markers,incidence of toxic side effects and long-term prognosis were compared between the two groups.Results The remission rate in group A was 82.75 ％ and the total effective rate was 100.00％;the remission rate in group B was 68.97％ and the total effective rate was 96.55％;there was no significant difference between the two groups (P＞0.05).After chemotherapy,serum CYFRA21-1 and SCCAg levels in patients of group A and B were significantly lower than those before chemotherapy (P＜0.05).During chemotherapy,the incidence of nausea,vomiting and diarrhea in group A was 17.24％ and 6.90 ％,respectively.The incidence of nausea,vomiting and diarrhea in group B was 48.28％ and 31.03 ％,respectively,the difference between the two groups was statistically significant (P＜0.05).After 36 months of follow-up,the overall survival rate in group A was 68.97 ％,and the overall survival rate in group B was 62.07 ％,there was no significant difference between the two groups (x2 =0.305,P =0.581);there was no significant difference in survival time between the two groups (Z=0.381,P=0.537).Conclusion The effect of Paclitaxel combined with lobaplatin in the preoperative neoadjuvant chemotherapy of LACC patients is equivalent to paclitaxel and cisplatin.

Chemical constituents from the roots tuber of Aconitum ouvrardianum / 中成药

AIM To study the chemical constituents from the roots tuber of Aconitum ouvrardianum H..METHODS The ethyl acetate fraction of methy extract from A.ouvrardianum roots tuber was isolated and purified by silica,aluminium oxide column,Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Eighteen compounds were isolated and identified as kongboendine (1),anisoezochasmaconitine (2),lipo-14-O-anisoylbikhaconine (3),franchetine (4),talatisamine (5),chasmanine (6),crassicauline A (7),chasmaconitine (8),14-dehydrotalatisamine (9),lipoindaconitine (10),indaconitine (11),yunaconitine (12),lipoyunaconitine (13),liljestrandisine (14),transconitine B (15),ouvrardiantine (16),atropurpursine (17),8-deacetylyunaconitine (18).CONCLUSION Compounds 1-4,9-10,13,15,17 are isolated from this plant for the first time.

Preparation and in vitro Drug Release of Betahistine Dihydrochloride Sustained-release Matrix Tablets / 中国药师

Objective: To prepare betahistine dihydrochloride sustained-release matrix tablets. Methods: The tablets were pre-pared with water soluble HPMC matrix, and the release behaviors were investigated by single factor study. The formula and preparation procedures were optimized by orthogonal design. Results:The optimal technology was as follows:using 60% HPMC K15M as the ma-trix material, calcium hydrogen phosphate as the filler, 10% PVP in 90% alcohol as the bonding agent;wet granulation compression technique was used to prepare the tablets with the tablet weight of 500mg. The in vitro drug release fits a Higuchi equation and the drug can be sustained-released within 12 h. Conclusion:The preparation technology is simple and the tablets have sustainol release behav-ior.