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Chinese Journal of Biotechnology ; (12): 557-564, 2012.
Artigo em Chinês | WPRIM | ID: wpr-342461

RESUMO

Acetylcholinesterase (AChE) plays a key role in the pesticide determination. However, the extraction of AChE from natural materials has the disadvantages of low yield, complex purification and poor stability. Therefore, the preparation of recombinant AChE with high performance becomes the hot topic of researchers in recent years. In this article we summarize the progress in the expression of recombinant AChE and the improvement of its analytical characteristic. Finally, we point out that the directed evolution strategy combined with surface display technology is the future trend on improving recombinant AChE activity.


Assuntos
Acetilcolinesterase , Química , Genética , Baculoviridae , Genética , Metabolismo , Técnicas de Visualização da Superfície Celular , Inibidores da Colinesterase , Evolução Molecular , Vetores Genéticos , Genética , Resíduos de Praguicidas , Pichia , Genética , Metabolismo , Proteínas Recombinantes , Genética
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