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Chinese Journal of Applied Physiology ; (6): 407-410, 2015.
Artigo em Chinês | WPRIM | ID: wpr-255002

RESUMO

<p><b>OBJECTIVE</b>To investigate the effect of component I from agkistrodon acutus venomon (AAVC-I) the migration of human umbilical vein endothelial cells (HUVECs), and to elucidate the possible anti-angiogenic mechanism of AAVC-I.</p><p><b>METHODS</b>The effect of AAVC-I on the migration of HUVECs which was cultivated in vitro and treated with AAVC-1 at four concentrations: 0, 20, 40, 80 microg/ml, was observed by methods of scratch wound-healing and Transwell assay. The expression level of mRNA and protein of P-selectin and intercellular cell adhension molecule-I (ICAM-1) were examined by RT-PCR and Western blot assay.</p><p><b>RESULTS</b>Compared with the blank group, the migration ability of HUVECs in each AAVE-I treated group was reduced in a dose-dependent manner, and the expression level of the mRNA and protein of P-selectin and ICAM-1 were decreased.</p><p><b>CONCLUSION</b>AAVC-I inhibits the migration of endothelial cell, which is acted by down-regulation of the expression content of mRNA and protein of P-selectin and ICAM-1.</p>


Assuntos
Humanos , Movimento Celular , Células Cultivadas , Venenos de Crotalídeos , Farmacologia , Regulação para Baixo , Células Endoteliais da Veia Umbilical Humana , Molécula 1 de Adesão Intercelular , Metabolismo , Selectina-P , Metabolismo , RNA Mensageiro
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