Epstein-Barr virus infection and expression of latent membrane protein 2A in Hodgkin's lymphoma of Uygur patients / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate Epstein-Barr virus(EBV) infection in Hodgkin's lymphoma (HL) of Uygur patients and related clinicopathological characteristics.</p><p><b>METHODS</b>EBV-encoded small RNA (EBER) was detected in 40 cases of HL and 20 cases of lymphoid reactive hyperplasia by in-situ hybridization. Expression of LMP2A in HL was investigated by immunohistochemistry.</p><p><b>RESULTS</b>EBV was detected in 26/40 (65.0%) of HL and 5/20 of lymphoid reactive hyperplasia (P < 0.05). The expression level of EBER showed significant difference among various histological subtypes of HL (P < 0.05) and between patients with and without B symptom (P = 0.02). However, no difference was found in relation to gender, clinical stage and tumor burden. The expression of LMP2A in the mixed cellularity and nodular sclerosis classical HL associated with EBV infection was 57.7% (15/26). Expression of LMP2A was not detected in lymphoid reactive hyperplasia cases.</p><p><b>CONCLUSION</b>Uyghur patients with Hodgkin's lymphoma have a high infection rate of EBV and distinct clinicopathologic characteristics.</p>

Differentially expressed proteins on postoperative 3 days healing in rabbit Achilles tendon rupture model after early kinesitherapy / 中华创伤杂志(英文版)

<p><b>OBJECTIVES</b>Surgical repair of Achilles tendon (AT) rupture should immediately be followed by active tendon mobilization. The optimal time as to when the mobilization should begin is important yet controversial. Early kinesitherapy leads to reduced rehabilitation period. However, an insight into the detailed mechanism of this process has not been gained. Proteomic technique can be used to separate and purify the proteins by differential expression profile which is related to the function of different proteins, but research in the area of proteomic analysis of AT 3 days after repair has not been studied so far.</p><p><b>METHODS</b>Forty-seven New Zealand white rabbits were randomized into 3 groups. Group A (immobilization group, n equal to 16) received postoperative cast immobilization; Group B (early motion group, n equal to 16) received early active motion treatments immediately following the repair of AT rupture from tenotomy. Another 15 rabbits served as control group (Group C). The AT samples were prepared 3 days following the microsurgery. The proteins were separated employing two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). PDQuest software version 8.0 was used to identify differentially expressed proteins, followed by peptide mass fingerprint (PMF) and tandem mass spectrum analysis, using the National Center for Biotechnology Information (NCBI) protein database retrieval and then for bioinformatics analysis.</p><p><b>RESULTS</b>A mean of 446.33, 436.33 and 462.67 protein spots on Achilles tendon samples of 13 rabbits in Group A, 14 rabbits in Group B and 13 rabbits in Group C were successfully detected in the 2D-PAGE. There were 40, 36 and 79 unique proteins in Groups A, B and C respectively. Some differentially expressed proteins were enzyme with the gel, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We successfully identified 9 and 11 different proteins in Groups A and B, such as GAPDH, phosphoglycerate kinase 1, pro-alpha-1 type 1 collagen, peroxiredoxin 1, alpha-1-antiproteinase E a-1 and MAD2L1 binding protein, etc. And some with the molecular chaperone, oxidative stress, energy metabolism, signal transduction, coupled with the tendon cell expression and protein synthesis, proliferate, differentiate and are closely related to the AT healing. The GAPDH protein was further validated through Western blotting. It was indicated that some differentially expressed proteins were involved in various metabolism pathways and may play an important role in initial healing of AT rupture.</p><p><b>CONCLUSION</b>Differentially expressed proteins in rabbit healing AT model may contribute to 3 days healing of AT rupture through a new mechanobiological mechanism due to the application of postoperative early kinesitherapy.</p>

Biomechanics during Achilles tendon healing / 中国组织工程研究

After Achilles tendon (AT) rupture operation,traditional gypsum immobilization must lead to AT in a condition of a tension-free or even "disuse"-negative balance healing system,which may inhibit the key opportunity of AT healing process to induce "active" mechanical signals stimulation,and AT "passively or intermittently" accepts the mechanical stimulation even applying the ultrasound,vibration and other mechanical stimulation to AT in the course of treatment. If using the method of active movement after operation,it can accelerate the AT healing,maintain function and promote re-construction of the AT,thereby help to restore the biomechanical properties of AT. Using proteomics techniques can reveal the mechanical signal transduction mechanisms of kinesiatrics promoting the AT healing in the high-throughput molecular level.

Correlation between clinical manifestation and salivary secretion of xerostomia patients / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To observe the general status, subjective syndromes, objective signs and salivary secretion of xerostomia patients for studying their correlations.</p><p><b>METHODS</b>Eighty patients' clinical data were collected and classified according to certain standards. The salivary secretion including the unstimulated whole saliva (UWS) and stimulated whole saliva (SWS) in five minutes was measured by spit method.</p><p><b>RESULTS</b>Decreased UWS was the main presentation of xerostomia which accouted for seventy five percents. Significantly statistical correlation were found between the decreased UWS and the dry mouth signs but not the age, sex and the accompany syndromes.</p><p><b>CONCLUSION</b>Xerostomia has a high prevalence in middle-aged women with a long course and obvious regularity. The decreased UWS is the main presentation which was not caused by physiological changes of salivary glands and has no correlation with many clinical manifestations.</p>