RESUMO
<p><b>OBJECTIVE</b>To explore a new method for the therapy of avascular necrosis of the femoral head.</p><p><b>METHOD</b>The recombinant plasmid pCD-rbFGF was mixed with collagen and was implanted in the necrotic femoral head. Expression of basic fibroblast growth factor (bFGF) was examined by RT-PCR and immunohistochemical method. Repair of the femoral head was observed by histological and histomorphometric analysis.</p><p><b>RESULT</b>Expression of bFGF was detected in the femoral head transfected with bFGF gene, indicating significant increase of angiogenesis 2 weeks after gene transfection and increased new bone formation 8 weeks after gene transfection on histomorphometric analysis (P< 0.01).</p><p><b>CONCLUSION</b>Transfection of bFGF gene enhances bone tissue angiogenesis. Repair in osteonecrosis would be accelerated accordingly.</p>
Assuntos
Animais , Coelhos , Colágeno , Usos Terapêuticos , Cabeça do Fêmur , Metabolismo , Necrose da Cabeça do Fêmur , Terapêutica , Fator 2 de Crescimento de Fibroblastos , Genética , Expressão Gênica , Terapia Genética , Neovascularização Fisiológica , Osteogênese , Plasmídeos , Proteínas Recombinantes , Genética , TransfecçãoRESUMO
<p><b>OBJECTIVE</b>To evaluate the effects of transforming growth factor beta1 (TGFbeta1) autocrine blockage on proliferation activity and drug sensitivity of osteosarcoma. METHODS; Northern blot, MTT determination, and 3H thymidine incorporation were used to investigate the effects of antisense TGF beta1 gene on osteosarcoma.</p><p><b>RESULTS</b>The proliferation of osteosarcoma cells transfected by antisense TGF beta1 gene was suppressed markedly, and adriamycin sensitivity was significantly increased.</p><p><b>CONCLUSION</b>Blockage of osteosarcoma cells TGF beta1 autocrine loop inhibits cell proliferation and enhances chemotherapy sensitivity.</p>
Assuntos
Humanos , Antineoplásicos , Farmacologia , Elementos Antissenso (Genética) , Genética , Comunicação Autócrina , Neoplasias Ósseas , Metabolismo , Patologia , Divisão Celular , Linhagem Celular Tumoral , Doxorrubicina , Farmacologia , Osteossarcoma , Metabolismo , Patologia , RNA Mensageiro , Genética , Transfecção , Fator de Crescimento Transformador beta , Genética , Fator de Crescimento Transformador beta1RESUMO
<p><b>OBJECTIVE</b>To elucidate the effects of exogenous basic fibroblast growth factor (bFGF) on biological characteristics of rat osteoblasts cultured in vitro.</p><p><b>METHODS</b>The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-beta(1)) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts.</p><p><b>RESULTS</b>bFGF (5-50 ng/ml) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-beta(1) mRNA increased significantly, but the intracellular ALP content decreased.</p><p><b>CONCLUSIONS</b>bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-beta(1), but cannot promote the differentiation of osteoblasts.</p>