RESUMO
OBJECTIVE:To establish HPLC method for the simultaneous content determination of metronidazole,tetra-caine hydrochloride and dexamethasone acetate in metronidazole compound.METHODS:The content determination was per-formed on column C 18 ;the mobile phase consisted methanol-water-triethylamine(65∶35∶0.36),the detection wavelength of both metronidazole and tetracaine hydrochloride was310nm,and240nm for dexamethasone acetate,the flow rate was1ml/min and the column temperature was25℃.RESULTS:The linear ranges of metronidazole,tetracaine hydrochloride and dexameth_ asone acetate were0.412?g～2.06?g(r=0.9999),0.191?g～0.956?g(r=0.9999)and0.121?g～0.604?g(r=0.9998),respectively,and their average recoveries were100.69%(RSD=1.28%),101.37%(RSD=0.23%)and102.40%(RSD=0.89%),respectively.CONCLUSION:The method was simple,accurate,reproducible,and suitable for the quality control of metronidazole compound.