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1.
Korean Journal of Nuclear Medicine ; : 152-162, 1999.
Artigo em Coreano | WPRIM | ID: wpr-186941

RESUMO

PURPOSE: To determine whether Tc-99mMIBI is recognized by the multidrug resistant P-glycoprotein (Pgp), we have measured quantitatively Tc-99mMIBI uptake in cancer cells. The effects of various Pgp reversing agents on cellular Tc-99m-MIBI uptake were also investigated in the presence of multidrug resistance gene-1 (mdr1 gene) overexpression. MATERIALS AND METHODS: We measured percentage uptake of Tc-99m-MIBI at different incubation temperatures both in mdr1 positive and negative cells. The effects of verapamil, cyclosporin, and dipyridamole on cellular uptake of Tc-99m-MIBI were also evaluated with or without overexpression of mdr1 gene in cultured murine leukemia L1210 cells. RESULTS: The mdr1 gene expressing cell lines were effectively induced in in vitro with continuous application of low-dose adriamycin or vincristine. Cellular uptake of Tc-99m-MIBI was higher in mdr1 negative L1210 cells than those of mdr1 positive cells, and higher when incubated in 37 degree C than 4 degree C. In the presence of verapamil, cyclosporin or dipyridamole, Tc-99m-MIBI uptake was increased upto 604% in mdr1 positive cells. CONCLUSION: Cellular uptake of Tc-99m-MIBI is lower in leukemia cells over-expressing mdr1 gene, and MDR-reversing agents increase cellular uptake. These results suggest that Tc-99m-MIBI can be used for characterizing Pgp expression and developing MDR-reversing agents In vitro.


Assuntos
Animais , Linhagem Celular , Ciclosporina , Dipiridamol , Doxorrubicina , Resistência a Múltiplos Medicamentos , Leucemia L1210 , Leucemia , Membro 1 da Subfamília B de Cassetes de Ligação de ATP , Tecnécio Tc 99m Sestamibi , Verapamil , Vincristina
2.
The Korean Journal of Thoracic and Cardiovascular Surgery ; : 654-660, 1993.
Artigo em Coreano | WPRIM | ID: wpr-189342

RESUMO

No abstract available.


Assuntos
Leiomiossarcoma
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