Investigation and Analysis of Hepatitis C Screened by Chemiluminescent Immu-noassay / 现代检验医学杂志

Objective The purpose of this study is to explore the applicability of chemiluminescent immunoassay in screening hepatitis C infection,and to find the infection status of heptitis C virus in Ningxia,and to provide scientific basis for its pre vention and control.Methods A total of 209 889 hepatitis C antibody (anti-HCV) data detected by chemiluminescent immu noassay (CIA) were collected from the General Hospital of Ningxia Medical University during 2011～2015.According to the 《Laboratory Hepatitis C Antibody Detection and Results Report Guide of 2003 CDC》of the United States,Cases were divid ed into weak positive (0.9≤S/CO value≤8.0) and positive (S/CO value＞8.0) two groups.According to the clinical diag nostic criteria of 《 Hepatitis C Prevention Guide》 of 2015 version to complete the diagnosis of acute and chronic hepatitis C.The chi square test and curve estimation of regression analysis was used to analyze the diagnosis rate of hepatitis C and the distribution characteristics of the patients with hepatitis C.Results In the S/CO value＞8.0 group,the diagnosis rate of hepatitis C was 97.57％.In the 0.9≤S/CO value≤8.0 group,the diagnosis rate of hepatitis C was only 2.05％.Among the people infected with HCV,there was no significant difference between male and female (x2 =1.432,P＞0.05).The infection rate was high in the individuals of ages between 21～60 years old,the infection rate of children and the elderly was low (x2 254.901,P＜0.01),almost all Departments of the hospital were distributed.The majority of false positive groups were elderly,children,pregnant women and tumor patients.The main departments were obstetrics,oncology,pediatrics and oral and maxillofacial surgery.Conclusion The method of Chemiluminescent immunoassay to detect anti-HCV is suitable for clinical screening of hepatitis C.but it is necessary to pay attention to the treatment of false positives.It is necessary to improve the detection of hepatitis C in the population with high prevalence in Ningxia,and pay attention to the prevention and treatment of nosoco-mial infection,and constantly improve the screening and monitoring system for hepatitis C.

Effect of etoposide on allergic contact dermatitis induced by dinitrofluorobenzene and its action mechanism in mice / 药学学报

This study is to observe the inhibition of etoposide on allergic contact dermatitis (ACD) and explore its possible mechanism of action. Dinitrofluorobenzene was used to induce the allergic contact dermatitis in mouse ear. Three groups of animals were orally administrated with different doses of VP-16 (5, 10, and 20 mg x kg(-1)), separately, for six days. The degree of skin inflammatory reaction was observed by optical microscope. Expression of intercellular adhesion molecule (ICAM-1) was detected by immunohistochemical staining. Radioimmunoassay was applied to measure the serum level of tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10). VP-16 significantly decreased inflammatory cell infiltration and the degree of infiltration reaction, and decreased the level of TNF-a in serum and the expression of ICAM-l in skin. VP-16 can significantly inhibit allergic contact dermatitis induced by DNFB. This therapeutic effect of VP-16 on murine ACD may be due to inhibiting expression of some cytokines.

Effect of baicalin on HSP70 expression of hippocampal neurons in focal brain ischemia-reperfusion injury rats / 药学学报

<p><b>AIM</b>To investigate the effect of baicalin on the hippocampal neuronal apoptosis and the expression of HSP70 in rats with focal brain ischemia-reperfusion injury.</p><p><b>METHODS</b>One hundred and twenty male Wistar rats were randomly divided into six groups:sham operated group, ischemia-reperfusion group, nimodipine group and three baicalin groups,to which baicalin was administered at doses of 50, 100 and 200 mg x kg(-1), separately. The models of focal brain ischemia-reperfusion injury induced by middle cerebral artery occlusion (MCAO) were used in this study. HE stain was used to observe the pathological changes. Flow cytometry (FCM) was used for determination of neuronal apoptosis. HSP70 protein expression of the neurons was detected with immunohistochemistry. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of the mRNA level of HSP70.</p><p><b>RESULTS</b>Baicalin can significantly relieve the pathological changes and inhibit apoptosis in hippocampus CA1 area, and at the same time increase the expression of HSP70 and HSP70 mRNA.</p><p><b>CONCLUSION</b>Baicalin can relieve brain damage induced by focal brain ischemia-reperfusion in rats, which may be related to inhibiting the process of the neuronal apoptosis. The mechanism of antiapoptosis effect of baicalin may be related to the promotion of transcription of HSP70 mRNA and increasing the expression of the protein.</p>

Studies on liver-toxicity in rhigoma of Dioscorea bulbifera / 中国中药杂志

<p><b>OBJECTIVE</b>To explore the liver-toxic fraction in Rhigoma of Dioscorea bulbifera.</p><p><b>METHOD</b>The rats were randomized into four groups: control group (20% PVP-water), T001(10% total methanol extraction), F002(5% chloroform fraction) and F003(5% methanol fraction). Direct bilirubin (DBil) and Glutamic-pyruvic transaminase (GPT) were examined, and liver index was measured. The histological and morphological observations were performed with optical and electrical microscope.</p><p><b>RESULT</b>T001 and F002 showed significant liver toxicity.</p><p><b>CONCLUSION</b>The chloroform fraction was the liver-toxic fraction of D. bulbifera.</p>