A new naphthalene derivative from bulbs of Eleutherine americana / 中国中药杂志

A new naphthalene derivative and three known compounds were isolated from the petroleum ether extract of the bulbs of Eleutherine americana by using various chromatographic techniques, such as column chromatography over silica gel and semi-preparative HPLC. Their structures were elucidated by spectroscopic date (MS, UV, IR, NMR), which were identified as eleutherol B (1), 4,8-dihydroxy-3-methoxy-1-methylanthraquinone-2-carboxylic acid methyl ester (2), 8-hydroxy-3,4-dimethoxy-1-methylanthraquinone-2-carboxylic acid methyl ester (3), and isoeleutherine (4). Compound 1 is a new compound. The diastolic blood vessels activity of compound 1 and 2 were potent, reaching 82.5% and 85.3% at the concentration of 10 μmol·L⁻¹, which were basically the same as that of the positive drug tanshinone ⅡA.

Studies on chemical constituents of Clinopodium chinense / 中国中药杂志

Twenty-eight compounds were isolated and purified from Clinopodium chinense by Sephedax LH-20, ODS, MCI and preparative HPLC. Their structures were identified as apigenin (1), apigenin-7-O-β-D-glucopyranoside (2), apigenin-7-O-β-D-glucuronopyranoside (3), thellungianol (4), apigenin-7-O-β-D-rutinoside (5), luteolin (6), luteolin-4'-O-β-D-glucopyranoside (7), apigenin-7-O-β-D-pyranglycuronate butyl ester (8), luteolin-7-O-β-D-rutinoside (9), luteolin-7-O-β-D-noehesperidoside (10), acacetin (11), acacetin-7-O-β-D-glucuronopyranoside (12), buddleoside (13), naringenin (14), pruning (15), nairutin (16), isosakuranetin (17), isosakuranin (18), didymin (19), hesperidin (20), kaempferol (21), quercetin (22), kaempferol-3-O-α-L-rahmnoside (23), p-hydroxycinnamic acid (24), caffeic acid (25), cis-3-[2-[1-(3,4-dihydroxy-phenyl)-1 -hydroxymethyl]-1,3-ben-zodioxol-5-yl]-(E)-2-propenoic acid (26), mesaconic acid (27), gentisic acid 5-O-β-D-(6'-salicylyl)-glucopyranoside (28). Among them, compounds 7, 9-10, 12, 23, 26-28 were isolated from the Clinopodium for the first time. The protective effects of compounds 1-6, 8-17 and 19 against H2O2-induced H9c2 cardiomyocyte injury were tested, compounds 15 exhibited significantly protective effects. Compared with the cell viability of (62.12±6.18)% in the model, pruning exhibited viabilities of (84.25±7.36)% at 25.0 mg•L⁻¹, respectively, using quercetin as a positive control [cell viability of (84.55±8.26)%, 20 mg•L⁻¹].

Cassane diterpenes from the seeds of Caesalpinia decapetala / 药学学报

Four casssane diterpenes were isolated from the ethanol extract of the seeds of Caesalpinia decapetala (Fabaceae), with a combination of various chromatographic approaches, including silica gel, Sephadex LH-20, reverse phase C18 and so on. On the basis of spectroscopic data analysis, they were identified as caesalpinin MQ (1), neocaesalpin N (2), caesalmin F (3) and α-caesalpin (4). Among them, compound 1 is a new diterpene, compounds 2-4 were isolated for the first time from the plant of C. decapetala.

Cassae-type diterpenes from seeds of Caesalpinia minax / 中国中药杂志

Fifteen cassaen-type diterpenes were isolated from the 95% ethanolic extract of the seeds of C. minax through various chromatographic techniques. Their structures were identified on the basis of spectroscopic data as pulcherralpin (1), caesalpinin ML (2), chamaetexane C (3), chamaetexane D (4), 6β, 18-diacetoxycassan-13, 15-diene (5), neocaesalpin K (6), neocaesalpin MP (7), neocaesalpin M (8), neocaesalpin Q (9), neocaesalpin P (10), neocaesalpin R (11), caesaldekarin D (12), caesaldekarin A (13), caesaldekarin b (14), 3β,6α-diacetoxyvouacapane (15). Among them, compounds 14, 9-11 were isolated from this plant for the first time.

Triterpene constituents from Rosa cymosa Tratt / 中国中药杂志

The constituents in 95% ethanol extract of the root of Rosa cymosa Tratt were purified by column chromatography techniques, leading to isolation of eleven triterpenes. Their structures were elucidated by spectroscopic data as pomolic acid (1), fupenzic acid (2), ursolic acid (3), euscaphic acid (4), arjunic acid (5), tomentic acid (6), 3β-E-feruloyl corosolic acid (7), 1β-hydroxyeuscaphic acid (8), myrianthic acid (9), cecropiacic acid (10), and ilexoside B (11). Among them, compounds 3, 6-8, 10 and 11 were obtained from this plant for the first time, and compounds 7 and 10 were obtained from this genus for the first time.

Review of studies on active components of antitumor medicinal plants / 中国中药杂志

This paper summarizes the current research on chemical structure, pharmacology and structure-activity relationship of antitumor constituents, including quinoline and indole alkaloids, stilbenoids, diterpenes, pentacyclic triterpenes, flavones, lactones, amyloses, proteins et al, of chinese herbal. The prospect of studies of antitumor medicinal plants is discussed.

Studies on chemical constituents from stems and leaves of Jasminum lanceolarium / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents in stem and leaves of Jasminum lanceolarium.</p><p><b>METHOD</b>The constituents of the EtOAc-soluble portion of the 95% ethanol extractive were isolated and purified by means of column chromatographic methods. Compounds were identified by their physical characteristics and spectral features.</p><p><b>RESULT</b>Eight compounds were isolated and identified as 5, 7, 3', 5'-tetrahydroxyflavanone (1), (2S)-5, 7, 3', 4'-tetrahydroxyflavan-5-O-beta-D-glucopyranosie (2), mannitol (3), nonacosane (4), trans-p-coumaric acid (5), cis-p-coumaric acid (6), ferulic acid (7) and, trans-cinnamic acid (8).</p><p><b>CONCLUSION</b>Compounds 14 were isolated from this genus for the first time. And compounds 5 and 6 were isolated from this plant for the first time.</p>

Effect of combined therapy with Sandostatin and yiyan mixture in treating severe acute pancreatitis / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the efficacy of combined therapy with Sandostatin (SDS) and Yiyan Mixture (YYM) in treating severe acute pancreatitis (SAP).</p><p><b>METHODS</b>Thirty-six patients with SAP were assigned to two groups equally, the treated group treated with the combined therapy and the control group treated by SDS alone. Biochemical indexes, including lactate dehydrogenase (LDH), aspartate aminotransferase (AST), amylase (AMY), endotoxin (ET), C-reactive protein (CRP), and APACHE II score were determined before and after treatment. And the incidence of complication and mortality were assessed.</p><p><b>RESULTS</b>The time of abdominal pain, tenderness and distention disappearance, air charge recovering, and average hospitalization days in the treated group were significantly shorter than those in the control group respectively (P < 0.05). Levels of LDH, AST, AMY, serum ET, CRP and APACHE II scores were lowered after treatment in both groups, but the decrement was more significant in the treated group than that in the control group respectively (P < 0.05). The difference in incidence of local complications and mortality between the two groups showed no statistical significance (P > 0.05).</p><p><b>CONCLUSION</b>Combined therapy of Sandostatin and YYM is a recommended recipe for treatment of SAP, it could obviously improve the condition of patients and enhance the efficacy of treatment.</p>

Studies on chemical constituents of Sarcandra glabra / 中国中药杂志

<p><b>OBJECTIVE</b>To study antibacterial chemical constituents of Sarcandra glabra.</p><p><b>METHOD</b>The constituents of the chloroform and EtOAc-soluble portions of the EtOH extract from the whole plant of S. glabra, which posses the antibacterial activities, were isolated and purified with column chromatography. The compounds were identified by physical and spectroscopic techniques.</p><p><b>RESULT</b>Six compounds were isolated and identified as 4, 4'-biisofraxidin (1), esculetin (2), fraxetin (3), scoparone (4), isofraxidin (5), scopoletin(6), respectively.</p><p><b>CONCLUSION</b>Compound 1 is a novel natural product. Compounds 24 were isolated from the plants of Chloranthaceae for the first time. The antibacterial activities of these six compounds were tested for the first time. Some compounds may have potential for future study and development as plant-derived oral antibacterial agents.</p>

Diterpenes from Helianthus annuus and their cytotoxicity in vitro / 药学学报

To search for bioactive compounds from the flower disc of Helianthus annuus L., chromatography was used to isolate and purify the chemical constituents, their structures were identified by spectral analysis, MTT method was applied to investigate their cytotoxic activities, some compounds showed moderate cytotoxic activities on SF-268, MCF-7 and HepG2 cell lines. Eleven compounds were obtained from the flower disc of H. annuus, and identified as ent-kaurane-2alpha, 16alpha-diol (1) and entkaurane-15alpha,16alpha-epoxy-17-al-19-oic acid (2), and nine known diterpenes, ent-kaurane-16P-ol (3), phyllocladan-16beta-ol (4) , ent-atisan-16a-ol (5) , grandifloric acid (6) , angeloylgrandifloric acid (7), ent-kaurane-16-en-19-oic acid (8), ent-kaurane-17-hydroxy-15-en-19-oic acid (9), ent-kaurane-16beta, 17-dihydroxy-19-oic acid (10), and ciliaric acid (11). Compounds 1 and 2 are new compounds, some compounds showed cytotoxic activities on SF-268, MCF-7 and HepG2 cell lines.

Studies on the chemical constituents in herbs of Hemistepta lyrata / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of Hemistepta lyrata.</p><p><b>METHOD</b>The constituents of the EtOAc-soluble portions of the 95% ethanol extract were isolated and purified by means of chromatography. Compounds were identified by their physical characteristics and spectral features.</p><p><b>RESULT</b>Five compounds were isolated and identified as caffeic acid (1), tracheloside (2), uracil (3), 8-carboxymethyl-p-hydroxycinnamic acid (4), and 3-O-p-coumaroylquinic acid (5).</p><p><b>CONCLUSION</b>Compounds 1-5 were isolated from this genus for the first time.</p>

Effect of Chinese herb Yiyanheji on intestinal mucosa barrier in rats with severe acute pancreatitis / 中国基层医药

Objective To study the effects of Chinese herbs Yiyanheji on intestinal mucosa barrier in rats with SAP.Methods Animal models of SAP were induced by retrograde injection of 5% sodium taurocholate (0.1ml/100g) into the common biliopancreatic duct.90 healthy Wistar rats weighing (250?30)g were randomly divided into 3 groups:sham operation group (SO,n=30),SAP group (SAP,n=30) and Chinese herbs Yiyanheji treated group(SAP+YH,n=30).Treated group was treated with Yiyanheji after operation.The SAP group was treated with physiological saline.Three groups of rats were killed at 72 hours after operation or treatment.Bacterial cultures were performed in all animals.The changes of terminal ileum' tissue were observed by optical electron mi- croscopy.The data of test were analyzed by statistic software.Results The incidence of bacterial translocation was 22.5% in Yiyanheji treated group,and it was lower than that in SAP group which was 90.0 %.The difference was significant(P

Isolation and structure determination of cypritibetquinone A and B, two new phenanthraquinones from Cypripedium tibeticum / 药学学报

<p><b>AIM</b>To study the chemical constituents of Cypripedium tibeticum.</p><p><b>METHODS</b>Compounds were isolated by repeated silica gel chromatography and purified on Sephadex LH-20 and structures were determined by spectral analysis.</p><p><b>RESULTS</b>Cypritibetquinones A and B were isolated from the ethyl acetate residue and their structures were determined as 7-hydroxy-2-methoxy-1 4-phenanthraquinone (1) and 7-hydroxy-2, 10-dimethoxy-l1 4-phenanthraquinone (2), respectively, by extensive spectral analyses.</p><p><b>CONCLUSION</b>Cypritibetquinones A and B are two new phenanthraquinones.</p>

A study on the rotation of crops among Panax quinquefolium, Perilla frutescens and Coix lacryma-jobi / 中国中药杂志

<p><b>OBJECTIVE</b>To provide evidence for establishing an efficient method of growing Panax quinquefolium by rotation of crops.</p><p><b>METHOD</b>Four-year old P. quinquefolium was cultivated in water and soil cultures. Biological assays were conducted with the aqueous extracts of P. quinquefolius, Fructus Perillae and roots of Coix lacryma-jobi. P. quinquefolium was cultivated in the soil where purple Perilla frutesens and C. lacryma-jobi were grown previously. The effects of rotation were determined.</p><p><b>RESULT</b>The stems, leaves and fibrous roots of Panax quinquefolium contained allelopathic substances. When the concentration of the allelopathic substances exceeded 1g per kg soil, P. quinquefolius could not grow. On the other hand, when the concentration of allelopathic substances fell below 0.2 g per kg soil, the rate of seedling growth was decreased by 25%. When P. quinquefolius was cultivated in the soil in which purple Perilla frutesens had previously grown, or in the soil supplemented with Fructus Perillae, the rates of seedling growth and the yield were raised by 26.8% and 11.5% tively, in comparison with the controls.</p><p><b>CONCLUSION</b>Rotation of Panax quinquefolium and Perilla frutesens was shown to be a good way for the cultivation of the former. During the growing process, application of Fructus Perillae to the soil could further enhance the growth and of Panax quinquefolium.</p>

Studies on chemical constituents in fruit of Rubus chingii / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of the fruit of Rubus chingii.</p><p><b>METHOD</b>The compounds were isolated and purified by recrystallization and chromatography with silica gel column and Sephadex LH-20 column. Their structures were identified by physicochemical properties and spectral analysis.</p><p><b>RESULT</b>Eleven compounds were isolated as oleanic acid (I), ursolic acid (II), maslinic acid (III), 2alpha-hydroxyursolic acid (IV), arjunic acid (V), hexacosyl p-coumarate (VI), tiliroside (VII), stearic acid (VIII), lacceroic acid (IX), beta-sitosterol (X), daucosterol (XI).</p><p><b>CONCLUSION</b>Compounds I, II, III, IV, V, VI, VIII, IX, XI were isolated from R. chingii for the first time.</p>

Study on chemical constituents in herbs of Anoectochilus roxburghii II / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Anoectochilus roxburghii.</p><p><b>METHOD</b>Silica gel and sephadex LH-20 column chromotography were used in the isolation from the ethanol extracts of the whole plant, the compounds were determined on the basis of various modern spectroscopic analysis and physical constants.</p><p><b>RESULT</b>Five compounds were isolated from the CHCl3 soluble portion, identified as p-Hydroxybenzaldehyde (I), ferulic acid (II), quercetin (III), daucosterol (IV), cirsilineol (V).</p><p><b>CONCLUSION</b>All these compounds were isolated from the plant for the first time, compound V was isolated from the Orchid Family for the first time, the other compounds were isolated from this genus for the first time.</p>

Chemical constituents in roots of Polygala fallax and their anti-oxidation activities in vitro / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in roots of P. fallax and their anti-oxidation activities in vitro.</p><p><b>METHOD</b>Column chromatographic techniques were employed for isolation and purification of chemical constituents of the plant. The structures were elucidated on the basis of the spectral evidence and the physical and chemical character. The isolated compounds were screened with four anti-oxidation models in vitro.</p><p><b>RESULT</b>Seven xanthones, 1,7-dihydroxy-2,3-methylenedioxyxanthone (1), 1-methoxy-2,3-methylenedioxyxanthone (2), 3-hydroxy-1,2-dimethoxyxanthone (3), 1,6,7-trihydroxy-2,3-dimethoxyxanthone (4), 7-hydroxy-1-methoxy-2,3-methylenedioxyxanthone (5), 1,3-dihydroxy-2-methoxyxanthone (6) and 1,3,7-trihydroxy-2-methoxyxanthone (7), were isolated from the roots of P. fallax. And compounds 1 - 7 showed different anti-oxidation activities in the different pharmacological models.</p><p><b>CONCLUSION</b>Compounds 2, 3, 5 and 7 were isolated from this plant for the first time. Xanthones from this plant showed anti-oxidation activities. The pharmacological activities of the pure compounds from this plant were also reported for the first time.</p>

Studies on chemical constituents in the seeds of Nigella glandulifera / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents in the seeds of Nigella glandulifera.</p><p><b>METHOD</b>The chemical constituents were isolated and repeatedly purified on silica gel column. They were identified and structurally elucidated by means of physio-chemical constants and spectral analysis.</p><p><b>RESULT</b>Nine compounds were identified as nigellamose (I), alpha-hederin (II), 3-O-[beta-D-xylopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinpyranosyl]-hederagenin (III), sucrose (IV), stearic acid (V), 1-O-hexadecanolenin (VI), beta-sitosterol (VII), daucosterol (VIII) and p-hydroxybenzoic acid (IX).</p><p><b>CONCLUSION</b>I, II, III, VI, VIII and IX were obtained from the plant for the first time, and I is a new compound.</p>

Studies on chemical constituents of sini tang / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of sini tang.</p><p><b>METHOD</b>The constituents were separated by means of column chromatography and their structures were identified by spectral data and compared with literature data.</p><p><b>RESULT</b>Six compounds were isolated and identified as vanillic acid, formononetin, isoliquiritigenin, liquiritigenin, kumatakenin B and uracil.</p><p><b>CONCLUSION</b>All of the compounds were isolated from sini tang for the first time and the study on their activities in Chinese prescription is being carried out.</p>

Studies on the chemistry of two new compounds of Hemistepta lyrata / 药学学报

<p><b>AIM</b>To study the chemical constituents of Hemistepta lyrata.</p><p><b>METHODS</b>To separate compounds with various chromatography technology and to elucidate their structures by chemical and spectral analysis.</p><p><b>RESULTS</b>Two compounds were isolated from Hemistepta lyrata and their structures were determined as hemiceramide (I), hemisterpene ether (II).</p><p><b>CONCLUSION</b>Compounds I and II are new compounds.</p>