RESUMO
Objective To establish the method for detemining the content of Baicalin in Ganteling Jiaonang by HPLC.Methods Using Diamonsil ODS1 C18 Column,methanol-water-phosphoric acid(50:50:0.2) as the mobile phase,detection wavelength as 280 nm and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.125 6～1.004 8 ?g for Baicalin and linear equation was Y= 95 952X+8 108.6,r2=0.999 7.The average recovery was 98.8% and RSD was 1.64%(n=6).Conclusion This method was simple,accurate,proper,and the reduplication of the result was good,which provide scientific quantitative analysis method for the determination of Baicalin in Ganteling Jiaonang.
RESUMO
Objective To establish the method for detemining the content of chlorogenic acid in Shuanghuanglian Keli by HPLC.Methods Using Diamonsil ODS1 C18 column,with methanol-water-glacial acetic acid(15:85:1) as the mobile phase,detection wavelength as 324 nm and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.060～1.210 ?g for chlorogenic acid and the equation was Y=105 427X+586.43,r2=0.999 5.The average recovery was 99.3% and RSD was 0.82%(n=6).Conclusion This method was simple,accurate and proper,and the reduplication of the result was good,which provide scientific quantitative analysis method of chlorogenic acid in Shuanghuanglian Keli.
RESUMO
Objective To establish the method for detemining the content of Icariin in Chuanlong Guci Pian by HPLC. Methods Diamonsil ODS1 C18 Column was used with acetonitrile-water (27∶73) as the mobile phase, detection wavelength as 270 nm, and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.125～1.00 ?g for Icariin and linear equation was Y=81 601X+10 356, r 2=0.999 7. The average recovery was 99.12% and RSD was 0.65% (n=6). Conclusion This method was simple, accurate and proper, and the reduplication of the result was good, which provides scientific quantitative analysis of Icariin in Chuanlong Guci Pian.
RESUMO
Objective To establish the method for detemining the content of Phillyin in Siji Ganmao Pian by HPLC.Methods Diamonsil ODS1 C18 column was used with acetonitrile-water(25∶75) as the mobile phase,detection wavelength as 277 nm and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.1128～0.9024 ?g for Phillyin and linear equation was Y =5.795 68X +1.843 19,r =0.999 3.The average recovery was 98.6% and RSD was 1.82%(n =5).Conclusion This method was simple,accurate and proper,the reduplication of the result was good.The method can be provide for quantitative analysis of Phillyin in Siji Ganmao Pian.
RESUMO
Objective To establish the method for determining the content of Fructus Cnidii in Dafeng wan by HPLC. Methods Diamonsil ODS1 C18 column was used with CH3OH-0.1 mol/L (NH4)2SO4 (70︰ 30) [adjust pH to 2.5 by sulphuric acid solution (1→5)] as the mobile phase. The detection wavelength was set at 322 nm and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.101～0.808 ?g for the Fructus Cnidii and linear equation was Y =9.964 406?10-3X -1.355 684?10-1, r=0.999 5. The average recovery was 98.4% and RSD was 1.40% (n=6). Conclusion This method was simple and accurate, with good specificity and repeatability, which provides scientific quantitative analysis method of Fructus Cnidii in Dafeng wan.