Comparative study of fatty acids and sterols of lipids of phalaris canariensis and echinochloa crusgalli

Gas liquid chromatography was used to determine the fatty acids and sterols composition of the lipid fractions obtained from Phalaris canariensis I and Echinochloa crusgalli II grains in different solvent extracts. The lipids obtained from both grains were significantly different in compositions for fatty acids and sterols contents

Urease in ulva lactuca chlorophyta: uvales

Urease [Urea amidohydrolase] was isolated from the green alga, Ulva lactuca L. and purified 127 fold by a procedure that included [NH[4]]2SO][4] fractionation and column chromatography using Sephadex G-200 followed by DEAE-cellulose. The preparation gave two bands when analysed by polyacrylamide gel electrophoresis. The enzyme had a pH optimum of 8.1 in Tris-HCl buffer at 30?C and required -SH groups for activity. Its molecular weight and apparent K[m]values were estimated to be 478600 and 238 uM respectively

Lipoxygenase from Ulva lactuca [Ubvales, chloropyta]

Lipoxygenase enzyme [linoleate oxygen oxidoreductase EC 1.99.2.1] was separated and purified from the green alga Ulvalactuca by extraction of the cell-free extract, precipitation with 45% to 70% solid ammonium sulfate and chromatography on DEAE Sephadex A 50. This preparation has given two bands [Lipoxygenase 1 and 2] by disc gel electrophoresis. Lipoxygenase enzyme from Ulva has been obtained in 60% yield with specific activity in the range of 1262 n mol, hydroperoxylinoleate mg[-1]protein. A purification 63-fold was achieved from Ulva. The optimum pH of Ulva lipoxygenase is 8.0. The molecular weights of lipoxygenase 1 and 2 were 120000 and 103000 respectively. K[m]and V [max] were 307.6 uM and 250 n mol per mg protein ml[-1]min[-1] respectively. No unusual amino acids were detected by the amino acid analyser. There is no cystine in Ulva lipoxygenase. The iron content of the purified lipoxygenase enzyme was 1.05 ppm

effect of salinity variations upon growth and organic solute contents of the marine green alga Enteromorpha intestinalis

Changes in simple organic compounds, namely soluble proteins, amino acids, soluble sugars and organic acids of the marine green alga Enteromorpha intestinalis have been examined in response to salinity variations. They were shown to vary in parallel to the external osmotic potential, thus, aiding the maintenance of hydration and turgor. However, the individual components of these organic solutes did not change in a similar manner. Proline, oxaloacetic and monosaccharides reached their maximum values at hypersaline media [45%], while the other individual fractions showed different behaviour, either slight increase or sharp decrease. Dry weight and protein content of the alga attained their maximum values at normal sea-water salinity [35%] which appears to be the most favourable one for growth of Enteromorpha intestinalis plant. At salinity 50%, all sugars, most of the amino acids and all the organic acids except oxaloacetic were at their minimum values