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1.
Chinese Journal of Pathology ; (12): 729-732, 2012.
Artigo em Chinês | WPRIM | ID: wpr-303477

RESUMO

<p><b>OBJECTIVE</b>To study the clinicopathologic features of primitive neuroectodermal tumor (PNET) in female genital tract.</p><p><b>METHODS</b>Six cases of PNET arising in female genital tract were retrospectively reviewed. The clinicopathologic features, immunohistochemical findings and EWS gene translocation study results were analyzed.</p><p><b>RESULTS</b>The age of patients ranged from 10 to 27 years (mean = 20 years). The sites of involvement included ovary (1 case), uterus (1 case), vulva (2 cases) and vagina (2 cases). The greatest diameter of the tumor ranged from 2 to 10 cm (mean = 5.4 cm). The tumor had nodular appearance and showed grayish-pink fleshy cut surface, accompanied by foci of hemorrhage and necrosis. Histologically, the tumor was composed of malignant small round cells with indistinct cell borders, hyperchromatic nuclei, dense chromatin, tiny nucleoli and scanty cytoplasm. The tumor cells were arranged in sheets or lobules. Homer-Wright rosettes were identified in 1 case. Immunohistochemical study showed that the tumor cells were positive for CD99, FLI-1 and CD56 (6/6). Focal expression of vimentin (5/6), NSE (5/6), nestin (4/6), synaptophysin (4/6), S-100 protein (2/6) and chromogranin A (1/6) was also demonstrated. EWS gene translocation was detected in 5 cases studied. Follow-up information was available in 2 patients (7 and 17 months of follow up, respectively). One of them died of tumor metastasis 17 months after diagnosis. The other patient was still alive.</p><p><b>CONCLUSIONS</b>PNET arising in female genital tract is rare. It mainly involves ovary, uterus, vulva and vagina. Immunohistochemical study using a panel of antibodies and fluorescence in-situ hybridization play an important role in definitive diagnosis of this rare malignancy.</p>


Assuntos
Adolescente , Adulto , Criança , Feminino , Humanos , Adulto Jovem , Antígeno 12E7 , Antígenos CD , Metabolismo , Antígeno CD56 , Metabolismo , Moléculas de Adesão Celular , Metabolismo , Seguimentos , Neoplasias dos Genitais Femininos , Genética , Metabolismo , Patologia , Cirurgia Geral , Tumores Neuroectodérmicos Primitivos Periféricos , Genética , Metabolismo , Patologia , Cirurgia Geral , Neoplasias Ovarianas , Genética , Metabolismo , Patologia , Cirurgia Geral , Proteína Proto-Oncogênica c-fli-1 , Metabolismo , Proteína EWS de Ligação a RNA , Genética , Estudos Retrospectivos , Translocação Genética , Neoplasias Uterinas , Genética , Metabolismo , Patologia , Cirurgia Geral , Neoplasias Vaginais , Genética , Metabolismo , Patologia , Cirurgia Geral , Vimentina , Metabolismo , Neoplasias Vulvares , Genética , Metabolismo , Patologia , Cirurgia Geral
2.
Chinese Journal of Pathology ; (12): 516-520, 2007.
Artigo em Chinês | WPRIM | ID: wpr-347743

RESUMO

<p><b>OBJECTIVE</b>To study the clinicopathologic features, diagnostic criteria, differential diagnosis and treatment options of ovarian steroid cell tumor, not otherwise specified (NOS).</p><p><b>METHODS</b>Light microscopy and immunohistochemical study was carried out in 8 cases of ovarian steroid cell tumor, NOS. The literature was reviewed.</p><p><b>RESULTS</b>The 7 cases of benign ovarian steroid cell tumor, NOS were composed mainly of polygonal cells with granular eosinophilic cytoplasm and larger cells with vacuolated cytoplasm. They resembled the architecture of normal adrenal gland, with formation of cell nests and trabeculae. The single case of malignant ovarian steroid cell tumor had evidence of significant cellular pleomorphism, haemorrhage and coagulative tumor necrosis. The mitotic count measured about 7 per 10 high-power fields. Immunohistochemical study showed that the tumor cells expressed calretinin and alpha-inhibin. Differential diagnosis included oxyphilic granulosa cell tumor, thecoma, Sertoli cell tumor and clear cell carcinoma. The treatment options of benign ovarian steroid cell tumor, NOS was local excision or ipsilateral salpingo-oophorectomy, while the malignant counterpart should be treated with a combination of surgery and chemotherapy, including administration of GnRH agonist.</p><p><b>CONCLUSIONS</b>Ovarian steroid cell tumor, NOS, is the most common type of ovarian steroid cell tumors. Most of which are associated with a benign clinical outcome. Immunohistochemistry is an important adjunct for diagnosis. The treatment options of ovarian steroid cell tumor, NOS depend on its malignant potential.</p>


Assuntos
Adolescente , Adulto , Feminino , Humanos , Adulto Jovem , Calbindina 2 , Diagnóstico Diferencial , Tumor de Células da Granulosa , Patologia , Inibinas , Metabolismo , Neoplasias Ovarianas , Metabolismo , Patologia , Cirurgia Geral , Ovariectomia , Métodos , Ovário , Patologia , Proteína G de Ligação ao Cálcio S100 , Metabolismo , Tumor de Células de Sertoli , Patologia , Tumores do Estroma Gonadal e dos Cordões Sexuais , Metabolismo , Patologia , Cirurgia Geral , Tumor da Célula Tecal , Patologia
3.
Chinese Journal of Medical Genetics ; (6): 258-260, 2005.
Artigo em Inglês | WPRIM | ID: wpr-321112

RESUMO

<p><b>OBJECTIVE</b>To investigate the possible association between ZNF230 gene and azoospermia.</p><p><b>METHODS</b>Screening for mutation of all 6 exons of ZNF230 gene was performed by denaturing high performance liquid chromatography(DHPLC) in 99 patients with azoospermia and in 115 healthy men as controls.</p><p><b>RESULTS</b>An A-->G transition at nucleotide 316 in exon 6 was identified. There were significant differences in the distribution profiles of both allele and genotype frequencies between patient group and control group (P < 0.01 and P < 0.05, respectively). In addition,there was a statistically significant difference in the serum follicle stimulating hormone (FSH) level between the patients with GG/GA genotype and those with AA genotype (P < 0.05).</p><p><b>CONCLUSION</b>ZNF230 gene may be associated with azoospermia, and the A316G mutation may be correlated with the serum FSH level.</p>


Assuntos
Adulto , Humanos , Masculino , Adulto Jovem , Azoospermia , Diagnóstico , Genética , Sequência de Bases , Cromatografia Líquida de Alta Pressão , Análise Mutacional de DNA , Proteínas de Ligação a DNA , Genética , Frequência do Gene , Testes Genéticos , Genótipo , Mutação , Reação em Cadeia da Polimerase , Fatores de Transcrição , Genética
4.
Chinese Journal of Oncology ; (12): 168-170, 2003.
Artigo em Chinês | WPRIM | ID: wpr-347468

RESUMO

<p><b>OBJECTIVE</b>To study the expression and significance of hypoxia inducible factor 1alpha (HIF-1alpha) in epithelial ovarian tumors.</p><p><b>METHODS</b>The expression of HIF-1alpha mRNA in 295 patients with epithelial ovarian tumor was analyzed retrospectively by high-throughput tissue microarray and in situ hybridization, which was compared with 13 normal ovarian tissue samples.</p><p><b>RESULTS</b>The expression rates of HIF-1alpha mRNA were 0, 13.2%, 42.1% and 81.9% in normal ovarian tissue, benign, borderline and malignant ovarian tumors. Expression rate of HIF-1alpha mRNA in borderline and invasive tumor was significantly higher than those in normal ovarian tissue and benign tumor (P < 0.001). Statistical analysis revealed that the expression of HIF-1alpha mRNA was not related to FIGO stages or histological subtypes. Close negative relation was observed between the expression of HIF-1alpha mRNA and tumor histological differentiation (P < 0.001).</p><p><b>CONCLUSION</b>The overexpression of HIF-1alpha may play an important role in oncogenesis of epithelial ovarian tumor. Tissue microarray is an efficient technique of molecular biology.</p>


Assuntos
Adulto , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , Subunidade alfa do Fator 1 Induzível por Hipóxia , Genética , Hibridização In Situ , Métodos , Neoplasias Epiteliais e Glandulares , Química , Metabolismo , Neoplasias Ovarianas , Química , Metabolismo , Ovário , Metabolismo , RNA Mensageiro , Análise Serial de Tecidos , Métodos
5.
Chinese Journal of Obstetrics and Gynecology ; (12)2001.
Artigo em Chinês | WPRIM | ID: wpr-683058

RESUMO

Objective To investigate the clinicopathology and immunophenotype of primary non- Hodgkin lymphoma(NHL)of the female genital system,and to analyze the prognosis of such tumors. Methods Clinicopathologic features of 43 cases of primary NHL of the female genital system were studied retrospectively,with the histological classification based on the Classification of Haematopoietic and Lymphoid Tumors(WHO,2001).Immunochemistry technique,in-situ-hybridization and polymerase chain reaction methods were used to detect the immunophenotype,epstein barrvirus(EB)virus infection status and immunoglobulin heavy chain gene rearrangement,respectively.Results(1)Primary lesions:there were 24 cases of lymphoma originating in the ovary,3 cases in the endometrium,10 cases in the cervix,2 cases in the vagina and 4 cases in the vulva.(2)Staging:12 cases(28%)were in stage Ⅰ,9 cases (21%)in stage Ⅱ,and 22 cases(51%)in stage Ⅲ.(3)Histological classification:37 cases(86%)were diffuse large B cell lymphoma(DLBCL),3 cases were Burkitt lymphoma and the remaining 3 cases were unspecified peripheral T-cell lymphoma according to biopsy,immunophenotype analysis,in-situ- hybridization technique and IgH gene rearrangement detection.(4)Prognosis analysis:increase in the level of lactic acid dehydrogenase,stage Ⅲ,DLBCL and single operation suggest poor prognosis.Conclusions Establishment of the diagnosis of primary NHL of the female genital system is based on biopsy, immunophenotype analysis,in-situ-hybridization technique and IgH gene rearrangement detection,which play important roles in diagnosis and differential diagnosis of the tumor.Combined therapy is the first choice of therapeutic regimens.

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