RESUMO
AIM The effects of misgurnus anguillicaudatus Polysaccharides(MAP) on the antihypoxia capacity in mice were studied. METHODS The mice were administered with various doses of MAP for 14 days and then were exposed to hypoxia. The survival time in mice, the content of hemoglobin (Hb), the content of MDA and the activity of superoxide dismutase (SOD) were examined. RESULTS MAP significantly prolonged the survival time in mice exposed to hypoxia. The optimal dose was 50 mg?kg -1 ?d -1 . MAP could inhibit lipid peroxidation in tissues, and increase Hb content and the activity of SOD in plasma. CONCLUSION MAP are the biological active components in Misgurnus anguillicaudatus against hypoxia.
RESUMO
A fungus that is capable of producing EPA and DHA was isolated from seawater. A fast extraction and methyl esterification of microbial lipid were established as well as their content determination by capillary gas chromatography using external calibration method. The results showed that the methyl ester prepared directly form the wet mycelia treated with CHCl3-KOH-CH3OH is less time-consuming and the method fits for the fast determination. To determine its EPA and DHA content,the wet mycelia was hydrolyzed according to Weibul 's method and the lipid obtained was methyl esteri.fied with KOH-MeOH,the results obtained by capillary gas chromatography using external calibration method were 7. 32% and 7. 58% respectively.
RESUMO
A fungus that is capable of producing EPA and DHA was isolated from seawater. A fast extraction and methyl esterification of microbial lipid were established as well as their content determination by capillary gas chromatography using external calibration method. The results showed that the methyl ester prepared directly form the wet mycelia treated with CHCl 3 KOH CH 3OH is less time consuming and the method fits for the fast determination. To determine its EPA and DHA content,the wet mycelia was hydrolyzed according to Weibul’s method and the lipid obtained was methyl esterified with KOH MeOH,the results obtained by capillary gas chromatography using external calibration method were 7.32% and 7.58% respectively.
RESUMO
Objective: Effects of cholesterol oxides (Ch-Ox) on plasma prostacyclin (PGI 2) and endothelin (ET) levels of rats in different selenium (Se) status were investigated. Methods: Weaning male Wistar rats were fed Se deficient diet (containing Se 0.038 mg/kg) and stock diet (Se 0.326 mg/kg, control group) respectively, for 13 weeks. One of Ch-Ox, cholestane-3 beta, 5 alpha, 6 beta-triol (3-triol) was injected into the rats through tail vein.24 h after treatment, the plasma PGI 2, thromboxane A 2 (TXA 2) and ET of animals were determined by radioimmunoassay. Results: Blood Se content, glutathione peroxidase activity and plasma PGI 2 levels of Se deficient rats were significantly lower than that of the control group (P