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Experimental & Molecular Medicine ; : 179-184, 2004.
Artigo em Inglês | WPRIM | ID: wpr-37850

RESUMO

Our aim was to apply DNA chip technology as a diagnostic tool in infertility research and clinics. Six loci, including a sex-determining region on the Y chromosome and five sequence-tagged sites in azoospermia-factor regions were investigated in infertile male patients. Our method produced a sensitive signal, which showed the presence or absence of the STS regions on the Y chromosome. The results from 93 patients with non- obstructive azoospermia, oligoathenoteratozoospermia, or oligozoospermia were identical when analyzed with either the DNA chip technique or conventional PCR-gel electrophoresis. We have demonstrated its application in the molecular diagnosis of male infertility. This system provides an economic and high-throughput method for detecting the deletion of genomic DNA sequences of large groups of infertile patients, and a completely new approach to male infertility screening. The application of DNA chip technology to identify Yq deletions can also facilitate our understanding of male infertility.


Assuntos
Feminino , Humanos , Masculino , Deleção Cromossômica , Cromossomos Humanos Y/genética , Análise Mutacional de DNA/métodos , Eletroforese em Gel de Ágar , Infertilidade Masculina/diagnóstico , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Reação em Cadeia da Polimerase , Valor Preditivo dos Testes , Proteínas de Plasma Seminal/genética , Sensibilidade e Especificidade , Sitios de Sequências Rotuladas , Aberrações dos Cromossomos Sexuais
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