Comparison of plasma adiponectin & certain inflammatory markers in angiographically proven coronary artery disease patients with & without diabetes – A study from India.

Background & objectives: The association between adiponectin and risk of cardiovascular disease is well known. The aim of the present study was to evaluate adiponectin and certain inflammatory markers and to determine the correlations between them in angiographically proven coronary artery disease (CAD) in subjects with and without diabetes. Methods: A total of 180 subjects who underwent coronary angiography for symptoms suggestive of CAD were categorised into groups based on their diabetes and/or CAD status: group1 (non-diabetic non-CAD); group2 (non-diabetic CAD); group3 (diabetic non-CAD) and group4 (diabetic CAD). Adiponectin, tumour necrosis factor α (TNF-α) and soluble form of E-selectin (sE-selectin) were estimated using quantitative sandwich enzyme immunoassay and high sensitive C-reactive protein (hsCRP) by particle enhanced immunoturbidimetric method. Results: Adiponectin levels were significantly lower in subjects with either diabetes or CAD and were much lower in subjects who had both. hsCRP was elevated in CAD and diabetes but did not differ significantly between groups. sE-selectin and TNF-α levels were elevated in CAD. Adiponectin negatively correlated with age, glucose, sE-selectin, total and LDL cholesterol. hsCRP correlated with BMI, sE-selectin and urea. sE-selectin correlated with BMI, triglycerides and VLDL cholesterol, whereas TNF-α correlated with fasting plasma glucose. In the logistic regression analysis, adiponectin had a significant inverse association with CAD. sE-selectin and TNF-α also showed significant independent association with CAD. Interpretation & conclusions: Adiponectin and other inflammatory markers such as sE-selectin and TNF-α showed a significant association with CAD. Hence, early assessment of such markers can help to identify high risk patients, and to reduce the inflammatory component of diabetes and CAD.

Risk factors associated with the development of overt nephropathy in type 2 diabetes patients: A 12 years observational study.

Background & objectives: Diabetic nephropathy (DN) is the leading cause of chronic kidney disease and end-stage renal disease in developing countries. Early detection and risk reduction measures can prevent DN. The aim of the study was to determine the risk factors for the development of proteinuria over a period of 12 years of follow up in normoalbuminuric type 2 diabetes patients attending a specialized centre. Methods: Of the 2630 type 2 diabetes subjects newly registered in 1996, 152 (M:F;92:60) normoalbuminuric subjects had baseline and subsequent measurements of anthropometric, haemodynamic and biochemical details spanning 12 years. The subjects were divided into 2 groups based on the renal status during follow up visits. Group 1 (non-progressors) had persistent normoalbuminuria and group 2 (progressors) had persistent proteinuria. Presence of other diabetic complications during follow up and details on antidiabetic and antihypertensive agents were noted. Results: During median follow up of 11 years in subjects with normal renal function at baseline, 44.1 per cent developed proteinuria at follow up. Glucose levels, HbA1c, systolic blood pressure (SBP), triglycerides, and urea levels were significantly higher at baseline among progressors than non-progressors. Progressors had a longer duration of diabetes and significant fall in estimated glomerular filtration rate (eGFR) levels at follow up. In Cox's regression analysis, baseline age, duration of diabetes, baseline HbA1c and mean values of HbA1c, triglycerides, SBP and presence of retinopathy showed significant association with the development of macroalbuminuria. Interpretation & conclusions: Type 2 diabetes patients with uncontrolled diabetes and increase in blood pressure are at high risk of developing nephropathy. Age, long duration of diabetes, elevated BP, poor glycaemic control and presence of retinopathy were significantly associated with the progression of diabetic nephropathy.

A pilot study on the effects of a polyherbal formulation cream on diabetic foot ulcers.

Background & objectives: Diabetic foot ulcers are the most common cause of non-traumatic lower extremity amputations in developing countries. The aim of this pilot study was to evaluate the safety of using a polyherbal formulation in healing diabetic foot ulcers in comparison with standard silver sulphadiazine cream among patients with type 2 diabetes. Methods: A total of 40 (M:F=29:14) consecutive type 2 diabetes patients with foot ulcers were enrolled in this study. They were randomly assigned to two groups of 20 each; Group 1 was treated with polyherbal formulation and group 2 with silver sulphadiazine cream. All the patients were followed up for a period of 5 months. The baseline ulcer size was noted and photograph of the wound was taken at the baseline and at each follow up visit. Number of days taken for healing of the wound was recorded. Results: The mean age of patients, duration of diabetes and HbA1c% were similar in both the study groups. The mean length and width of the ulcers was also similar in both the groups at baseline visit. There was a significant decrease in the size of the wound (length and width) in both the study groups (P<0.001). The mean time taken for the healing of the ulcer was around 43 days in both groups. Interpretation & conclusions: Diabetic wound cream prepared by using polyherbal formulation was found to be effective as well as safe in healing diabetic foot ulcers like the standard silver sulphadiazine cream.

Efficacy of Glycated Albumin (GA) in Comparison with Glycated Haemoglobin (HbA1c) in Type 2 Diabetic Subject in India.

The study was planned to determine the efficacy of GA in comparison with HbA1c among type2 diabetic subjects. A total of 94 type 2 diabetic (group2) subjects were selected for this prospective study and were compared with 50 non diabetic controls (group1). The subjects were reviewed for a period of 3months. Anthropometric, blood pressure, plasma glucose, GA and HbA1c measurements were done at baseline, 1st and final follow up visits for all the subjects.GA levels strongly correlated with HbA1c% both in control and study group. The mean GA and HbA1c values were significantly lower at the baseline and during follow up visits in controls than in study subjects (p<0.001). GA, HbA1c and the ratio decreased significantly within 4weeks, but GA showed a significantly larger decrease than HbA1c. There was no significant difference in the GA% after 3months. GA may be a useful marker for assessing short term glycemic changes in type2 diabetes.

Limited joint mobility and plantar pressure in type 1 diabetic subjects in India.

AIM: Limited joint mobility and plantar pressure in the foot has not been assessed in type 1 diabetes. The aim of this study was to investigate the joint mobility and plantar foot pressure in Asian Indian type 1 diabetic subjects and to see its association with duration of diabetes. MATERIAL AND METHODS: The joint mobility and plantar pressure were measured in 115 consecutive subjects attending the foot clinic. The study groups were: Control- non diabetic controls (n=40) (M:F 19:21) and type 1 diabetic patients (n=75) (M:F 42:33). Joint mobility was assessed using a goniometer at two sites, in the subtalar joint and in the hallux. Plantar pressure was measured using the RS-Scan platform system. Data obtained on the metatarsal heads were used for analysis. RESULTS: Patients with type 1 diabetes had significantly lesser joint mobility (p < 0.0001) and higher plantar pressure (p < 0.0001) compared with the control group. Duration of diabetes had an inverse association with joint mobility (p < 0.0001). The degree of joint mobility was more restricted in patients with longer duration of diabetes. Plantar pressure increased with increasing duration of diabetes. CONCLUSION: In conclusion, type 1 diabetic patients in India had limited joint mobility which decreased further with longer duration of diabetes and they had high plantar pressure also.