Genotype characterization of occult hepatitis B virus strains among Egyptian chronic hepatitis C patients

Chronic hepatitis C virus [HCV] infection combined with occult hepatitis B virus [HBV] infection has been associated with increased risk of hepatitis, cirrhosis and hepatocellular carcinoma. This study aimed to determine the prevalence of occult HBV infection among Egyptian chronic HCV patients, the genotype and occurrence of surface gene mutations of HBV and the impact of co-infection on early response to treatment. The study enrolled 162 chronic HCV patients from Ismailia Fever Hospital, Egypt, who were HBV surface antigen-negative. All patients were given clinical assessment and biochemical, histological and virological examinations. HBV-DNA was detectable in sera from 3 patients out of the 40 patients who were positive for hepatitis B core antibody. These 3 patients were responsive to combination therapy at treatment week 12; only 1 of them had discontinued therapy by week 24. HBV genotype D was the only detectable genotype in those patients, with absence of [a] determinant mutations among those isolates

Phenotypic characterization of Acinetobacter baumannii isolates from intensive care units at a tertiary-care hospital in Egypt

Multi-drug resistant [MDR] strains of Acinetobacter baumannii are responsible for an increasing number of opportunistic infections in hospitals. This study determined the prevalence of MDR A. baumannii isolates from intensive care units in a large tertiary-care hospital in Ismailia, Egypt, and the occurrence of different beta-lactamases in these isolates. Biotyping and antimicrobial susceptibility profile was done for isolated strains. Respiratory, urine, burn wound and blood specimens were collected from 350 patients admitted to different units; 10 strains [2.9%] of A. baumanniiwere isolated. All isolates showed resistance to more than 3 classes of antibiotics. Among the isolates, 6 isolates were carbapenemase producers, 2 were AmpC beta-lactamase producers and no isolates were metallo-beta-lactamase producers. Despite the low prevalence of A. baumannii infection in this hospital, the antibiotic resistance profile suggests that prevention of health-care-associated transmission of MDR Acinetobacter spp. infection is essential

Detection of methicillin-resistant Staphylococcus aureus directly by loop-mediated isothermal amplification and direct cefoxitin disk diffusion tests

We evaluated the utility of 2 methods for detection of methicillin-resistant Staphylococcus aureus [MRSA] directly from signal-positive blood culture bottles: loop-mediated isothermal amplification [LAMP] assay, and direct cefoxitin disk diffusion [DCDD] test using a 30 microg cefoxitin disk. In parallel, standard microbiological identification and oxacillin susceptibility testing with MecA PCR was performed. Of 60 blood cultures positive for Gram-positive cocci in clusters, LAMP [via detection of the FemA and MecA genes] showed 100% sensitivity and specificity for identification of MRSA/MSSA. When coagulase-negative staphylococci were tested, sensitivity for detection of methicillin resistance was 91.7% and specificity was 100%. DCDD along with direct tube coagulase assay detected only 80.6% of MRSA/MSSA. LAMP showed higher diagnostic accuracy although DCDD was more cost-effective and did not require additional reagents or supplies

High prevalence of Klebsiella pneumoniae carbapenemase-mediated resistance in K. pneumoniae isolates from Egypt

The emergence and rapid spread of antibiotic-resistant Klebsiella pneumoniae isolates harbouring the bla[KPC] gene that encodes for carbapenemase production have complicated the management of patient infections. This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the bla[KPC] gene and compared the results with modified Hodge test. Antibiotic sensitivity was performed by standard methods, and interpreted following both the old CLSI breakpoints [M100-S19] for carbapenems and the revised breakpoints [M100-S22]. From the 45 non-duplicate isolates of K. pneumoniae recovered from different clinical specimens, a high prevalence of ertapenem-nonsusceptible isolates [44.4%] was reported using the new lower CLSI breakpoints. The bla[KPC] gene was confirmed in 14/20 [70.0%] of these isolates. The high prevalence of ertapenem nonsusceptibility at a tertiary care hospital in Egypt was predominantly attributed to K. pneumoniae carbapenemase-mediated resistance mechanisms in K. pneumoniae isolates

Development of -a basic membrane protein gene- targeted nested PCR Assay for the direct detection of T. pallidum DNA in specimens from patients with suspected syphilis

We report the development of a nested-PCR-based assay targeting the basic membrane protein gene for the detection of T. pallidum DNA in specimens from patients with genital ulcer disease. The specificity and sensitivity of the test were assessed. The detection limit is down to 28 copies per reaction when analyzed on gel. The technique was then applied to genital ulcer samples from 51 patients with suspected syphilis, who were also tested by serological methods. We obtained positive reactions for 15 specimens out of 16 specimens from patients with primary and secondary syphilis and negative results for all the non-syphilis group. Nested PCR is here compared with other diagnostic methods currently used in diagnosis of venereal syphilis. PCR successfully detected T. pallidum from oral, genital, and anal ulcers present during primary or secondary syphilis and the results correlated well with the serology