Effect of diazinon and / or praziquantel on selected protein aspects in healthy and Schistosoma mansoni infected mice

The effect of 1/20 LD50 diazinon given orally to healthy and Schistosomiasis mansoni infected mice for 5-9 successive days and 17 weeks coupled with a therapeutic dose [2x500 mg/kg b. Wt.] of praziquantel two weeks before sacrificing. The results showed insignificant differences obtained from total proteins, albumin, globulins and albumin/globulin ratio. However, significant differences were revealed from alpha 1, alpha 2, beta 1, beta 2 and gamma-globulins in addition to plasma ceruloplasmin. Diazinon changed the levels of alpha 2, beta 1 and gamma-globulins, while diazinon coupled with schistosomiasis affected the levels of most of the studies parameters

Biochemical changes after subchronic and chronic interaction of Schistosoma mansoni infection in Swiss albino mice with two specific compounds

The effects of diazinon [DZN] and/or praziquantel [PZQ] on the levels of plasma triiodothyronine [T3] and thyroxine [T4], activities of brain acetylcholinesterase [AchE] and liver alanine aminotransferase [ALT] in addition to blood reduced glutathione [GSH] in healthy and Schistosoma mansoni [Sm] infected mice were investigated after 9 and 17 weeks of either infection or intoxication with DZN. Triiodothyronine showed significant differences among the different treatments. The group of mice treated with PZQ showed the highest levels of T3 at both time intervals. Thyroxine level showed significant differences between the two time intervals. The lowest levels of T4 were observed in the infected-PZQ grown at week 17. The maximum inhibition of brain AchE activity was noticed in DZN-PZQ treated group after 9 and 17 weeks

Schistosoma mansoni: IgG reactivity to soluble adult worm antigen preparation [swap] in sera of immunized mice with irradiated attenuated and virulent cercariae

The aim of this work was to identify the antigens, that elicit a greater or unique immune response in the immunized host against Schistosoma mansoni [Egyptian strain] infection. Moreover, the difference in immune response to this antigen between mice immunized with radiation-attenuated cercaria or immunized with virulent cercaria were investigated. Immunoblotting technique was used to monitor the fine specificity of host IgG to SDS-PAGE separated SWAP in the sera of different test groups immunized with either radiation-attenuated cercaria or low doses of virulent cercaria compared with non-immunized group