Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom

Abstract Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents. Methods Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Student’s t-test. The significance level was set at p< 0.05. Results It was observed that H. lunatus venom increased heart rate and caused arrhythmia, thereby impairing the heart functioning. Lungs of envenomed animals showed significant alterations, such as diffuse hemorrhage. In addition, immunofluorescence showed that H. lunatus venom was capable of binding to cardiomyocytes. Furthermore, mouse ventricular cardiomyocytes incubated with H. lunatus venom showed a significant decrease in calcium transient, confirming that H. lunatus venom exerts a toxic effect on heart. Conclusion Our results showed that H. lunatus venom is capable of inducing cardiorespiratory alterations, a typical systemic effect of scorpionism, stressing the importance of medical monitoring in envenomation cases.

Cardiorespiratory alterations in rodents experimentally envenomed with Hadruroides lunatus scorpion venom

Abstract Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents. Methods Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Students t-test. The significance level was set at p 0.05. Results It was observed that H. lunatus venom increased heart rate and caused arrhythmia, thereby impairing the heart functioning. Lungs of envenomed animals showed significant alterations, such as diffuse hemorrhage. In addition, immunofluorescence showed that H. lunatus venom was capable of binding to cardiomyocytes. Furthermore, mouse ventricular cardiomyocytes incubated with H. lunatus venom showed a significant decrease in calcium transient, confirming that H. lunatus venom exerts a toxic effect on heart. Conclusion Our results showed that H. lunatus venom is capable of inducing cardiorespiratory alterations, a typical systemic effect of scorpionism, stressing the importance of medical monitoring in envenomation cases.

Nursing Activities Score e carga de trabalho em unidade de terapia intensiva de hospital universitário / Nursing Activities Score and workload in the intensive care unit of a university hospital

Objetivo: A carga de trabalho de enfermagem é constituída pelo tempo dispendido pela equipe de enfermagem para realizar as atividades de sua responsabilidade, relacionadas direta ou indiretamente ao atendimento do paciente. O objetivo deste estudo foi avaliar a carga de trabalho de enfermagem em uma unidade de terapia intensiva adulto de hospital universitário com o uso do instrumento Nursing Activities Score (NAS). Métodos: Estudo longitudinal, prospectivo, envolvendo pacientes admitidos na unidade de terapia intensiva de um hospital universitário no período de março a dezembro de 2008. Foram coletados dados para o cálculo do NAS, do Acute Physiology and Chronic Health Evaluation (APACHE II), do Sequential Organ Failure Assessment (SOFA) e do Therapeutic Intervention Scoring System (TISS-28), diariamente até a saída da unidade de terapia intensiva adulto ou 90 dias de internação. O nível de significância adotado foi de 5%. Resultados: Foram avaliados 437 pacientes, resultando em NAS de 74,4%. O tipo de internação, tempo de permanência na unidade de terapia intensiva e condição de saída do paciente da unidade de terapia intensiva e do hospital foram variáveis associadas a diferenças na carga de trabalho da enfermagem. Houve correlação moderada do NAS médio com o escore de gravidade APACHE II (r=0,329), com o escore de disfunção orgânica SOFA médio (r=0,506) e com o TISS-28 médio (r=0,600). Conclusão: Encontramos elevada carga de trabalho de enfermagem no estudo. Esse resultado pode subsidiar planejamento para dimensionamento da equipe. A carga de trabalho sofreu influência de caraterísticas clínicas, sendo observado aumento do trabalho nos pacientes cirúrgicos de urgência e nos não sobreviventes. .

Objective: The nursing workload consists of the time spent by the nursing staff to perform the activities for which they are responsible, whether directly or indirectly related to patient care. The aim of this study was to evaluate the nursing workload in an adult intensive care unit at a university hospital using the Nursing Activities Score (NAS) instrument. Methods: A longitudinal, prospective study that involved the patients admitted to the intensive care unit of a university hospital between March and December 2008. The data were collected daily to calculate the NAS, the Acute Physiology and Chronic Health Evaluation (APACHE II), the Sequential Organ Failure Assessment (SOFA) and the Therapeutic Intervention Scoring System (TISS-28) of patients until they left the adult intensive care unit or after 90 days of hospitalization. The level of significance was set at 5%. Results: In total, 437 patients were evaluated, which resulted in an NAS of 74.4%. The type of admission, length of stay in the intensive care unit and the patients' condition when leaving the intensive care unit and hospital were variables associated with differences in the nursing workload. There was a moderate correlation between the mean NAS and APACHE II severity score (r=0.329), the mean organic dysfunction SOFA score (r=0.506) and the mean TISS-28 score (r=0.600). Conclusion: We observed a high nursing workload in this study. These results can assist in planning the size of the staff required. The workload was influenced by clinical characteristics, including an increased workload required for emergency surgical patients and patients who died. .

Concepções de desenvolvimento e de aprendizagem no trabalho do professor / Development and learning conceptions regarding teachers work

Esta pesquisa teve por objetivo identificar, explicitar e analisar as concepções de desenvolvimento/aprendizagem presentes no trabalho de profissionais de uma escola pública, visando à construção de conhecimentos que permitissem transformar a prática educativa. Elaboraram-se roteiros de entrevista para os professores, o coordenador pedagógico e a direção, um roteiro de observação de aulas e um para consulta ao projeto pedagógico. Os dados foram organizados em categorias que relacionam as concepções de desenvolvimento/aprendizagem apresentadas pela Teoria Histórico-Cultural àquelas encontradas nos dados obtidos na escola. Verificou-se que o trabalho desses profissionais não é fundamentado em uma única concepção de desenvolvimento/aprendizagem, o que pôde ser demonstrado pela coexistência de várias teorias, entre as quais se destacam a de Piaget e a de Vigotski. Constatou-se que as abordagens dos profissionais da escola são permeadas pela cotidianidade, discutindo-se, a partir da Teoria do Cotidiano, de Heller, a invasão de um espaço de produção do conhecimento por ações/reflexões cotidianas.

The purpose of this research was to identify, explain and analyze, the conceptions of development and learning that are present in the work of professionals working for a public school, aiming at constructing a type of knowledge able to transform educational practices. Besides a class observation itinerary and norms to consult the pedagogical project, norms in relation to interviews - to be applied to the teachers, the pedagogical coordinator and the principal - were elaborated. The data were organized into categories, connecting the development and learning conceptions - presented by the Historical-cultural-theory - to the theories, emerging in the data obtained in the school analyzed. It was verified that the work of those professionals is based, not only in one development or learning theoretical conception. In fact, it was observed the coexistence of many theories, such as the ones conceived by Piaget and Vigotski. The results show that, the school professional approaches are permeated by everyday life, and the invasion of a space for knowledge production is discussed based on actions and reflections related to everyday life.

Esta investigación tuvo el objetivo de identificar, explicitar y analizar las concepciones de desarrollo / aprendizaje presentes en el trabajo de profesionales de una escuela pública, visando la construcción de conocimientos que permitieran transformar la práctica educativa. Se elaboraron guías de entrevista a los profesores, al coordinador pedagógico y a la dirección, una guía de observación de clases y otra para la consulta al proyecto pedagógico. Los datos fueron organizados en categorías que relacionan las concepciones de desarrollo / aprendizaje presentadas por la Teoría Histórico-cultural a aquellas encontradas en los datos obtenidos en la escuela. Se verificó que el trabajo de esos profesionales no es fundamentado en una única concepción de desarrollo / aprendizaje, lo que puede ser demostrado por la coexistencia de varias teorías, entre las cuales se destacan la de Piaget y la de Vygotsky. Se constató que los enfoques de los profesionales de la escuela son permeadas por la cotidianidad, discutiéndose, a partir de la Teoría del Cotidiano, de Heller, la invasión de un espacio de producción del conocimiento por acciones/ reflexiones cotidianas.

A produção do conhecimento psicológico-psiquiátrico em saúde mental: considerações a partir de um texto exemplar / The psychological production and psychiatric knowledge in mental health: considerations from an exemplar text

Este trabalho discute a relação entre ideologia e o conhecimento produzido na Psiquiatria, a fim de desvelar o que está oculto em um texto considerado científico. O material analisado trata da prevenção primária de distúrbios psíquicos que produzem “desajustamento social”. A análise centra-se no próprio conceito de prevenção como ideologia, numa perspectiva crítica que busca as lacunas que garantem e legitimam o poder político dos que detêm o conhecimento hegemônico na área da saúde mental