RESUMO
Objective: To assess the effect of combination of pulsed electromagnetic fields [PEMF] with predatory stress on transected sciatic nerve regeneration in rats
Methods: In sham- operated group [SOG] the nerve was manipulated and left intact. The 10-mm rat sciatic nerve gap was created in rats. In transected group [Transected] nerve stumps were sutured to adjacent muscle and in vein graft group [VG] the gap was bridged using an inside-out vein graft. In VG/PEMF group the transected nerve was bridged using vein graft, phosphate buffered saline was administered into the graft and the whole body was exposed to PEMF. In VG/PS group the transected nerve was bridged using vein graft, phosphate buffered saline was administered into the graft and the rats underwent predatory stress [PS]. In VG/PEMF/PS group the transected nerve was bridged using vein graft, phosphate buffered saline was administered into the graft, the whole body was exposed to PEMF and the rats underwent predatory stress. The regenerated nerve fibers were studied within 12 weeks after surgery
Results: Functional, gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in VG/PEMF and VG/PEMF/PS groups compared to those in the other groups [p=0.001]. The whole body exposure to PEMF improved functional recovery. Predatory stress did not affect nerve regeneration in the animals undergone predatory stress [p=0.343]
Conclusion: Pulsed electromagnetic fields could be considered as an effective, safe and tolerable treatment for peripheral nerve repair in clinical practice
RESUMO
Introduction and Aims: Ruta Graveolens [RG] has been used in various medical preparations for its anti-oxidant, anti-inflammatory, anti-tumour, anti-androgenic and anti-fertility properties. More than 120 natural compounds mainly including acridone alkaloids, coumarines, essential oils, flavonoids, and furoquinolines have been found in the roots and aerial parts of this plant. The aim of this work is to study the anti-fertility propertiese of RG extract. Histochemical studies have been carried out in mouse ovary
Materials and Methods: In this study 36 female mice were used in two groups as control and RG. Control group received the saline normal 0.2 ml and the RG group received 300mg/kg of the aqueous extract of RG per day orally for 14 days. Ovaries were studied after staining ALP, PAS and sudan Black
Results: It was found that in RG group of first week most follicle were atretic. Results indicate that the staining intensity in ALP, PAS and Sudan Black were sever in RG group when compared with the control group. There was no significant difference between the control and RG group after 2 and 3 weeks treatment
Conclusion: RG extract caused metabolic disorder and reduced fertility. This may be due to the increase in the intensity of reaction due to the accumulation of lidids and carbohydrates and damage of intracytoplasmic organels
RESUMO
Objective: To assess the neuroprotective effects of local administration of 17- beta- estradiol on nerve regeneration
Methods: Sixty female Wistar rats were overiectomized and divided into four experimental groups [n = 15], randomly: In autograft group a segment of sciatic nerve was transected and re-implanted reversely. In sham-surgery group sciatic nerve was exposed and manipulated. In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group defect was bridged using a silicon conduit filled with 10 microL [0.1 mg/mL] 17- beta- estradiol. Each group was subdivided into four subgroups of five animals each and nerve fibers were studied in a 12-week period
Results: Behavioral, functional, biomechanical, electrophysiological and gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in treatment group than in other groups [p<0.05]. Immunohistochemical reactions to S-100 in treatment group were more positive than that in other groups
Conclusion: Local administration of 17-beta-estradiol improved functional recovery and morphometric indices of sciatic nerve. It could have clinical implications for the surgical management of patients after facial nerve transection
RESUMO
AbstrnetBackground: One of the affecting factors in disturbance process of spermatogenesisis chemotherapeutic-induced oxidative stress resulted from cyciophosphamide [CP]; treatment which leads to diminisled sperm quality via interference in spermatogenesis process. Objective: This study was conducted to investigate the effects of ethyl pyruvat [EP] in reducing the CP-induced side effects on reproductive system.Materials and Methods: 24 mature male mice were randomly divided into 3 equal j groups and were undergone therapy for 35 days. Control group received normalsaline [0.1 mliday, IF]. CP group were injected CP [15 mgikglweek, IP] and CP+EPgroup received EP [40 mgilcglday, IP] as well as CP. In the end of the treatment: period, the mice were euthanized by cervical dislocation. Then, the epididymis wasincubated with C02 in a human tuba1 fluid medium [1 ml] for half an hour in order: to float sperm. Then, the number, motility, viability [eosin-nigrosin staining], DNAbreakage [acridine orange staining], nucleus maturity, and sperm morphology I [aniline blue staining] were analyzed. Results: The average [15.871-1.28], motility [35.77+2.75], viability [4013.03], I nucleus maturity [36*2.79] and sperm morphology [61.751-0.85] were decreasedI significantly in CP group in comparison with control and EP groups, whereas I caused significant increase of these parameters. Also, the percentage of DNA I damage was increased significantly in CP group [41.75*3.75] in comparison with I control [2*0.71] and EP groups [22.514.13]. Conclusion: The results of this study revealed ameliorating effects of EP on sperm quality of CP treated animals