Post-transcriptional protein modification of Gata4 / 中国当代儿科杂志

Gata4 is an important transcription factor in heart development. Gata4 post-transcriptional protein modification regulates transcriptional activity and DNA binding, which in turn affects expression of downstream genes and transcription factors, differentiation of embryonic stem cells and cardiogenesis. This article summarizes the effect of post-transcriptional protein modification on transcriptional activity of Gata4 and the relationship between this effect and congenital heart disease. It was shown that acetylation, phosphorylation and SUMOylation upregulate transcriptional activity, DNA binding, downstream gene expression and embryonic stem cell differentiation. On the other hand, methylation and deacetylation downregulate Gata4 transcriptional activity. Post-transcriptional protein modification of Gata4 is very important in clinical research on congenital and other heart diseases.

Approaches of strengthening innovation and efficiency in ideological and political education among university students / 上海第二医科大学学报

Objective To analyze the effects and experience of ideological and political education through inquiring students' satisfaction on it,and to strengthen innovation and efficiency. Methods Five hundred and fifty university students were chosen by random.Through anonymous questionnaires,information on attention and satisfaction of ideological and political education about educate by all staff,campus culture,psychological status,and extracurricular activities were acquired.Results More than 56% of the students thought who impressed them most were the professional teachers;and 75.64% of them would ask friends for help when they were facing difficulties.And 44.91% considered study as the main resource of pressure,although the other 39.09% chose the career perspective.Then,57.45% were willing to take part in the meaningful activities after class,though the study was tough.And 45.45% hoped it would be a great progress on extracurricular activities;74% would take part in entertainment,and on the other hand 68.55% chose volunteer;and 87.72% interested in learning psychological knowledge. Conclusion It is important to strengthen and develop ideological and political education among students in the university.And attention should be paid to the innovation and efficiency of the education.

Analysis of prognostic and clinicopathologic factors in gastrointestinal stromal tumors of the stomach / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To identify the clinical pathological characteristics and prognostic factors in patients with gastrointestinal stromal tumors of the stomach.</p><p><b>METHODS</b>The data of 98 patients of gastric stromal tumors, leiomyomas, leiomyosarcomas, leiomyoblastomas, schwannomas and neurofibromas, collected from Mar. 1983 to Dec. 2001 in our hospital with complete clinical and pathological data, were investigated retrospectively. Gastric stromal tumors were diagnosed by reviewing the tumor slides stained with hematoxylin and eosin (H&E). Two histomorphologically representative areas of each tumor slides were identified and arrayed on a tissue microarray. Immunohistochemistry staining were performed using antibodies to c-kit (CD117), CD34, smooth muscle actin (SMA), Desmin and S-100 proteins. The relations of various clinicopathologic characteristics and outcomes were tested by univariate analysis and multivariate analysis.</p><p><b>RESULTS</b>Ninety-one patients were clearly identified as gastric stromal tumors from the 98 patients, who were diagnosed as gastric stromal tumor, leiomyoma, leiomyosarcoma, leiomyoblastoma schwannoma and neurofibroma (92.9%). The follow-up rate was 91% and the median follow up time was 54 months. The patient survival rates at 1, 5 and 10 years were 88.8%, 79.6% and 63.7% respectively. Univariate analysis showed that tumor size, mitotic count, tumor necrosis, nuclear pleomorphism, cell type, cell density, surgical procedure, mucosal invasion, age and lable index of Ki-67 were associated with prognosis (P<0.05). Multivariate analysis showed that tumor size, mitotic count, mucosal invasion and tumor necrosis were predictors of prognosis (P<0.05).</p><p><b>CONCLUSION</b>Tumor size of >10 cm, mitotic count of >10 mitoses per 50 high power fields, necrosis and mucosal invasion are often associated with an aggressive clinical course in gastric stromal tumors.</p>

Intra-abdomen extragastrointestinal stromal tumors: a clinicopathologic study on 30 cases / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To investigate the clinicopathological characteristics and prognostic factors in patients with intra-abdomen extragastrointestinal stromal tumors (EGISTs).</p><p><b>METHODS</b>The data of 47 patients of mesenchymal neoplasms that arose from the abdominal cavity and retroperitoneum, collected from July 1987 to June 2003 in our hospital with complete clinical and pathological data, were investigated retrospectively. EGISTs were diagnosed by reviewing the tumor slides stained with hematoxylin and eosin (H&E). Immunohistochemistry staining were performed on CD117, CD34, smooth muscle actin, Desmin and S-100 proteins. The relations of various clinicopathologic characteristics and outcomes were examined.</p><p><b>RESULTS</b>Among the 47 cases, 30 tumors were confirmed to be EGISTs. Twelve cases arose from the mesentery, six from small omentum, eight from retroperitoneum and four from the abdominal cavity. The size of tumors ranged from 4 to 30 cm (median 12.5 cm) in diameter and the tumor cell components mainly included spindle cells (23 cases), epithelioid cells (4 cases), and mixed cells (3 cases). The follow-up rate was 90% and the median follow up time was 44 months. The patient survival rates at 1, 5 and 10 years were 79.7%, 59.5% and 45.4% respectively. Univariate analysis showed that tumor size >10 cm, tumor necrosis, mitoses > or =5/50HPF, obvious nuclear atypia, moderate and poor differentiated tumor cells were predictors of poor prognosis.</p><p><b>CONCLUSIONS</b>EGISTs have specific clinical behaviors. The parameters used for predicting GISTs prognosis are not completely applicable for EGISTs. Tumor necrosis, obvious nuclear atypia and mitoses > or =5/50HPF help to predict aggressive behaviors in EGISTs.</p>