Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation

PURPOSE: To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation. METHODS: Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity. RESULTS: A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate. CONCLUSION: Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation. .

Trauma da queimadura em crianças e suas implicações nutricionais / Burns trauma in children and its nutritional implications

Objetivo: Descrever particularidades relativas à nutrição em pacientespediátricos com queimaduras e a adesão à prescrição dietética no primeiro centro especializado do Pará. Método: Foram inclusos pacientes de zero a dez anos, internados no Centro de Tratamento de Queimados do Hospital Metropolitano de Urgência e Emergência do Estado do Pará. Os procedimentos de avaliação antropométrica, diagnóstico nutricional, estimativa das necessidades nutricionais, valor nutritivo das dietas oferecidas, adesão às dietas hospitalares, uso e indicação de suporte nutricional e tratamento estatístico foram cumpridos. Resultados: Um total de 49 pacientes foram estudados. As queimaduras observadas chegaram a comprometer até 53% da superfície corporal. Eutrofia esteve presente em mais de 50% da amostra. O estado nutricional foi inversamente proporcional ao tempo de internação hospitalar (p=0,0001). A dieta hospitalar atendeu às necessidadesde 97,95% dos pacientes em relação a calorias e 100% a proteínas, de acordo com a fórmula preditiva de necessidades nutricionais. Cerca de 88% das necessidades de suplementos foram cumpridas. Anorexia foi detectada em 71,43% da amostra, sendo observada em 100% dos pacientes com superfície corporal queimada acima de 21% (p<0,005). Conclusão: A manutenção do estado nutricional se mostrou importante para a recuperação do estado clínico. Apesar de a dieta hospitalar e o uso de suplementos atenderem às necessidades nutricionais da maioria dos pacientes, a anorexia limitou as possibilidades da terapia nutricional oral, comprovando a necessidade de estimular o uso de sondas e nutrição enteral neste centro de queimados.

Objective: To describe particularities related to nutrition in pediatric patients with burns and the adherence to dietary prescription in the first specialized center in the Para. Methods: We included patients from zero to ten years, admitted to the Burn Treatment Center ofMetropolitan Hospital of Urgency and Emergency State of Para. Methods:Procedures anthropometric, nutritional diagnosis, estimation of nutrient requirements, nutritional value of diets offered, adherence to hospital diets, requirements and use of nutritional supplements and statistical analysis were co™mpleted. Results: A total of 49 patients were studied. The total body surface area stricken represents 53%.Normal weight was present in more than 50% of the sample. Nutritional status was inversely proportional to the length of hospital stay (p<0.0001). The hospital diet attended the requirements of 97.95% of patients in terms of calories and protein to 100% according to the formula predictive of nutritional needs. About 88% of supplements needs are met. Anorexia was detected in 71.43% of the sample, being observed in 100% of patients with total body surface area over 21% (p <0.005). Conclusion: The maintenance of nutritional status was important for the recovery of clinical table. Although thehospital diet and use supplements meet the nutritional needs of most patients, anorexia has limited the possibilities of oral nutritional therapy, confirming the need to encourage the use of enteral nutrition in the place of study.