An Early Year History of Biological Preparation of Silver Nanoparticles in West Bengal and their Antibacterial Activity: A Review

Biologically prepared silver nanoparticles are in trend tobe used as antibacterial agents throughout the globe. Silvernanoparticles prepared from different biological sources havebeen tested against Staphylococcus aureus, Escherichia coli,and other clinical bacteria in West-Bengal also. The size, shapeand activity of the biogenic silver nanoparticles will varydepending upon the biological sources and its concentrationused for nanoparticle preparation. UV-Vis spectrophometry,Dynamic light scattering, FESEM, HRTEM are the techniqueswhich can be used for characterizing silver nanoparticles ofdifferent size and shape. From the history of last decade ofresearch upon silver nanoparticles’ green synthesis and itsantibacterial, antifungal, antilarval as well as anticancer agents,researchers used plant parts, fungus and bacteria as biologicalsources for the reduction of silver ions to silver nanoparticles.Which showed promising activity against different bacterialstrains, either procured from ATCC (American type culturecommittee) or from any clinical sources. When it comes toanalyse the activity of the prepared silver nanoparticles againstmultidrug-resistant (MDR) clinical bacterial strains, there arelesser evidences from West-Bengal. This review will work asa reservoir for biologically prepared silver nanoparticles inWest-Bengal in the last decade and will also help researchersto characterize biogenic silver nanoparticles

Antiserum directed against cell surface antigens is lethal to Leishmania donovani promastigotes.

The purified flagellar fraction of Leishmania donovani promastigotes consists of 30-35 polypeptides. Antiserum raised against this fraction reacts with both flagella and pellicular membrane antigens as evident from immunoblot and immunofluorescence studies. Only 3 of these immunoreactive polypeptides are flagellum-specific. The antiserum agglutinates the cells and inhibits their growth in liquid culture medium. Moreover, glucose uptake and glucose-stimulated oxygen uptake of the promastigotes are significantly inhibited by the antiserum. The results indicate that the antiserum has a profound lethal effect on the in vitro propagation of the parasite.