Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Adicionar filtros








Intervalo de ano
1.
Chinese Journal of Biotechnology ; (12): 590-594, 2004.
Artigo em Chinês | WPRIM | ID: wpr-270081

RESUMO

In silkworm moth the colleterial gland markedly enlarged due to the secretion and accumulation of glue like substances before adult emergence. However, the Ng mutant female moth only secreted little glue-like substance and laid loose eggs naturally. In the present experiment, it was extracted the proteins of secretory part of the variety E981 and its Ng mutant line and analyzed by two-dimensional electrophoresis. More than 700 protein spots were resolved both in two samples and most of the proteins were distributed in the area from 30 kD to 70 kD and pH 4 - 8. Through the comparison and analysis, it was found that 4 proteins were only expressed in E981 and 2 proteins were only expressed in Ng mutant. Furthermore, there are about 29 proteins were expressed higher in 981 and about 15 proteins expressed volume were higher in Ng mutant. These differential proteins may be have some relations with the Ng mutant form and directly lead to the Ng mutant can't secret the glue-like substance.


Assuntos
Animais , Feminino , Bombyx , Metabolismo , Eletroforese em Gel Bidimensional , Glândulas Exócrinas , Química , Proteínas de Insetos
2.
Chinese Journal of Biotechnology ; (12): 352-355, 2002.
Artigo em Chinês | WPRIM | ID: wpr-256204

RESUMO

A 44-residue hybrid peptide (CB (1-24)-Arg-Ser-Tyr-Tan (4-21)) incorporating 1-24 residues of cecropin B (CB) and 4-21 residues of thanatin (Tan) was designed and constructed. The CB-Tan gene was cloned into expression plasmid pGEX-3X and expressed in E. coli BL21. The fusion protein was purified by affinity chromatography. After digested with enterokinase the gene product released with antibacterial activity and gave one band in Tricine-SDS-PAGE.


Assuntos
Sequência de Aminoácidos , Antibacterianos , Metabolismo , Peptídeos Catiônicos Antimicrobianos , Sequência de Bases , Clonagem Molecular , Escherichia coli , Genética , Proteínas de Insetos , Dados de Sequência Molecular , Peptídeos Cíclicos , Proteínas Recombinantes de Fusão , Farmacologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA