RESUMO
Objective @#To observe the expression of Galectin⁃3 in peritoneal dialysis ( PD) fluid in patients with different dialysis ages , and to conduct correlation analysis with vascular endothelial growth factor ( VEGF) , fibronectin (FN) and related clinical indicators . @*Methods @#A total of 109 PD patients who were regularly followed up in the department of nephrology were divided into four groups according to different peritoneal dialysis ages . The concentrations of Galectin⁃3 , VEGF and FN were determined by enzyme⁃linked immunosorbent assay . The expression of Galectin⁃3 in peritoneal dialysate of the 4 groups was compared , the correlation with VEGF , FN and clinical related indexes was analyzed , and the correlation was analyzed by Spearman test . @*Results @# The concentration of VEGF in peritoneal dialysis patients in group D significantly increased ( P < 0. 05 ) . Galectin⁃3 expression levels were positively correlated with VEGF ( r = 0. 358 , P = 0. 022) , but not significantly correlated with FN ( r = 0. 121 , P = 0. 452) . Galectin⁃3 was positively correlated with clinical indicators parathyroid hormone (PTH) ( r = 0. 201 , P = 0. 037) , C ⁃reactive protein (CRP) ( r = 0. 357 , P < 0. 001) , left ventricular posterior wall dimensions ( LVPWD) ( r = 0. 213 , P = 0. 026) , and negatively correlated with clinical indicators total cholesterol (TC) ( r = - 0. 316 , P = 0. 001) . @*Conclusion @#The concentration of Galectin⁃3 in the dialysate of long⁃term peritoneal dialysis patients is significantly elevated , indicating that the expression of galectin⁃3 increases with the extension of peritoneal dialysis time , suggesting that the detection of galectin⁃3 levels may be helpful for the evaluation of early peritoneal fibrosis . The positive correlation with VEGF may suggest its role in promoting peritoneal angiogenesis and fibrosis . Moreover , it is positively correlated with clinical indicators PTH , CRP and LVPWD , suggesting that it has certain clinical guiding significance on microinflammatory state and myocardial remodeling .
RESUMO
Objective @#To study the effect and mechanism of high glucose on mesothelial-mesenchymal transition (MMT) of peritoneal mesothelial cells (HMrSV5) , and the protective effect of pharmacological blocking of signal transducer and activator of transcription 3 (STAT3) on rat peritoneal fibrosis (PF) model . @*Methods @#The animals were divided into three groups : the sham group , the model group , and the STAT3 inhibitor group . A miniature per- itoneal dialysis catheter was implanted under the dorsal skin of rat and the rat peritoneal fibrosis model was induced by daily injection of high glucose dialysate . After 10 weeks , HE staining was used to evaluate the histology of the peritoneum , and the level of transforming growth factor-β1 (TGF-β1) in the peritoneum was measured by immuno- histochemistry . HMrSV5 was cultured in high glucose and the optimal stimulation concentration of high glucose was determined by Western blot. High glucose was used to stimulate HMrSV5 after successful transfection with si - STAT3 and Western blot was used to measure the protein level of STAT3 , p-STAT3 , and the key enzymes of glycol- ysis 6-phosphofructo-2-kinase/fructose-2 , 6-biphosphatase 3 (PFKFB3) and lactate dehydrogenase A (LDHA) .@*Results @#HE staining showed that administration of STAT3 inhibitor ( BP-1-102) could inhibit the thickening of subperitoneal tissue and the proliferation of vessels in HG dialysis rats . The expression of TGF-β1 in the rats perito- neum of the model group was significantly higher than that in the sham group , and the level of TGF-β1 was marked- ly lower in the STAT3 inhibitor group compared to the model group (P < 0. 05) . Compared to the control group , high glucose induced the up-regulation of α-smooth muscle actin ( α-SMA) , the down-regulation of E-cadherin and STAT3 activation in HMrSV5 (P < 0. 05) . Mesothelial cells treated with high glucose also exhibited high expres- sion of the key enzymes of glycolysis ( PFKFB3 , LDHA) ( P < 0. 05) , and si-STAT3 can effectively inhibit the overexpression of PFKFB3 and LDHA induced by high glucose ( P < 0. 05) . @*Conclusion @#STAT3 is involved in high glucose-induced HMrSV5 hyperglycolysis and MMT , and targeting STAT3 alleviates peritoneal fibrosis and an- giogenesis during peritoneal dialysis treatment in rats .