Inhibitory Activity of Porcine Placenta Extracts on Angiotensin I Converting Enzyme / 日本補完代替医療学会誌

<i>Objective</i>: Angiotensin I Converting Enzyme was used to study the inhibitory activity of porcine placenta extracts.<br> <i>Methods</i>: ACE was derived from rabbit lung. Bz-Gly-His-Leu was used as a substrate. Production of hippuric acid was measured by HPLC.<br> <i>Result</i>: ACE inhibitory activity was measured in a two-fold dilution of porcine placenta extracts. The IC₅₀ of porcine placenta extracts was 95.7 μg. The results of a Lineweaver-Burk plot suggested that porcine placenta extracts exhibited a competitive inhibition.<br> <i>Conclusion</i>: In this paper, we found that porcine placenta extracts had an anti-ACE activity.<br>

Suppression of Proliferation of Prostate Cancer Cells and Benign Prostatic Hyperplasia by Porcine Placenta Extracts / 日本補完代替医療学会誌

<i>Objective</i>: Anti-androgenic action of porcine placenta extracts were investigated.<br> <i>Methods</i>: To determine if porcine placenta extracts can inhibit androgen-stimulated growth of LNCaP cells, a dose response assay was performed. LNCaP cells were treated with varying concentrations of porcine placenta extracts with or without dihydrotestosterone.<br> A castrated rat was used to study influence of an exogenous androgen.<br> <i>Results</i>: Porcine placenta extracts-treated cells showed a statically significant decrease in cell number, indicating decreased cell growth.<br> The secretion of PSA(prostate specific antigen) was determined by ELISA. It was also inhibited in the presence of porcine placenta extracts and dihydrotestosterone.<br> Immunoblotting detected decreases in androgen receptor protein after treatment with the porcine placenta extracts.<br> In vivo studies in a castrated rat showed that enlargement of the prostate by testosterone was inhibited by porcine placenta extracts.<br> <i>Conclution</i>: These results suggest that porcine placenta extracts contain a substance with anti-androgenic actions.<br>