RESUMO
<p><b>OBJECTIVE</b>To investigate the correlation between atorvastatin inhibiting the expression level of matrix metalloproteinase 9 (MMP-9) and peroxisome proliferator activated receptor alpha (PPARalpha) signal channel in myocyte of aging rat.</p><p><b>METHODS</b>Primary cultures of myocyte were got ten from aging rats. Myocyte were divided into control group, DMSO group, atorvastatin group, atorvastatin plus GW6471 group, which treated respectively by cell culture medium, DMSO, atorvastatin, atorvastatin plus GW6471. The expression of MMP-9 mRNA was evaluated by RT-PCR, and content of MMP-9 protein was detected by Western blot.</p><p><b>RESULTS</b>(1) There was no difference between control group and DMSO group in level of MMP-9 mRNA and protein expression (P > 0.05); (2) The level of MMP-9 mRNA and MMP-9 protein expression in atorvastatin group were significantly lower than those in control group (P < 0.01); (3) Both level of MMP-9 mRNA and protein expression in atorvastatin plus GW6471 group were significantly higher than those in atorvastatin group (P < 0.05), but were still lower than those in control group (P < 0.05).</p><p><b>CONCLUSION</b>Atorvastatin inhibit MMP-9 expression of aging myocytes by PPARalpha signal channel.</p>
Assuntos
Animais , Ratos , Envelhecimento , Atorvastatina , Células Cultivadas , Ácidos Heptanoicos , Farmacologia , Metaloproteinase 9 da Matriz , Metabolismo , Células Musculares , Metabolismo , Oxazóis , Farmacologia , PPAR alfa , Metabolismo , Pirróis , Farmacologia , Ratos Wistar , Transdução de Sinais , Tirosina , FarmacologiaRESUMO
<p><b>OBJECTIVE</b>To study EEG during simulated flight training, then to explore the situational character of mental fatigue of the pilots.</p><p><b>METHODS</b>Fifty male pilots were randomly choice to be recorded their EEG including rest,flight and recovery phase. Then the band waves of EEG were analysed among the three phase.</p><p><b>RESULTS</b>(1) During flight, the amplitude of alpha, beta, SMR, theta, gamma waves tended to increase. These changes seemed obviously in SMR, theta waves. (2) After flight, all the amplitude of the waves declined except for beta wave of the right lobe,and most of them were still higher than those before flight (P < 0.05); (3) The right brain showed a higher cerebration than the left, especially in theta wave.</p><p><b>CONCLUSION</b>Mental fatigue is started during the flight training, and the right lobe has a severe mental fatigue than the left. After flight, mental fatigue showed a slow recovery.</p>
Assuntos
Adulto , Humanos , Masculino , Medicina Aeroespacial , Eletroencefalografia , Fadiga Mental , Educação Física e TreinamentoRESUMO
<p><b>OBJECTIVE</b>In this study, we pretreated the mice ASMCs by dexamethasone (Dex) within 10 min, to test the peak of [Ca2+]i and phospho-PLCbeta (ser1105) in the cells by treated with Ach.</p><p><b>METHODS</b>The peak of [Ca2+]i was measured by Fura-2/AM methods and the phospho-PLCbeta-ser1105 was by Western blot, and compared with dexamethasone pretreated groups. Glucocorticoid receptor antagonist RU486 and the protein synthesis inhibitor cycloheximide groups were settled in our study.</p><p><b>RESULTS</b>Glucocorticoids (GCs) significantly decreased the resting values and peak of [Ca2+]i elevation and elevated the intracellular levels of phospho-PLCbeta (ser1105) in 10 min. Neither the RU486 nor cycloheximide could alter the inhibitory effects of glucocorticoids stated above.</p><p><b>CONCLUSION</b>Our results demonstrate that glucocorticoids exert rapid inhibitory effects. The series of signal changes in this process that restrain the peak of [Ca2+]i may be responsible for the rapid nongenomic inhibitory effects of GCs by reducing the activity of PLC.</p>