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Journal of Biomedical Engineering ; (6): 898-901, 2007.
Artigo em Chinês | WPRIM | ID: wpr-346046

RESUMO

This study was aimed to work out a simple, applicable, sensitive and specific protocol for the identification of phosphoproteome. Isotope-labeling, two-dimensional electrophoresis, autoradiography and so on were used to establish a phosphoproteome map of mice neurons, and then chemiluminescence Western blotting was utilized to detect three phosphoproteins PI3Kr3, MEK1 and PKCalpha selectively. The results of comparison showed that the blots of PI3Kr3, MEK1 and PKCalpha on autoradiography map were almost identical with the blots of PI3Kr3, MEK1 and PKCalpha on chemiluminescence Western blotting maps. So this protocol based on isotope labeling and chemiluminescence Western blotting methods has proven to be sensitive and specific in the identification of phosphoproteome.


Assuntos
Animais , Camundongos , Animais Recém-Nascidos , Western Blotting , Métodos , Encéfalo , Biologia Celular , Células Cultivadas , Eletroforese em Gel Bidimensional , Métodos , Medições Luminescentes , Métodos , Neurônios , Química , Biologia Celular , Fosfoproteínas , Radioisótopos de Fósforo , Proteoma , Sensibilidade e Especificidade
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