RESUMO
Based on the purpose of conservation planning for native species, sixteen populations of cattle, goat and sheep were analyzed by amplification of genomic DNA using inter-simple sequence repeat [ISSR] markers to estimate of genetic structure. DNA samples of 275 animals were collected to Paccess their genetic content. The polymorphism information content [PIC] values and genetic diversity in sheep populations were higher than the others. The mean coefficient of gene differentiation [Gst] was 0.3615, indicating that 19.42% of the genetic diversity resided within the population. In total, 60 fragments in PCR products were indicated by using ISSR primers and generally most of the fragments were common in all populations, but differed in their frequency. A cluster analysis was carried out using unweighed pair group method with arithmetic averages [UPGMA] and dendrogram illustrated genetic relationships among 275 individuals in three species. Haplotypes were constructed computationally and frequencies were compared in each species. The results of this study can provide basic molecular information for future research on native livestock using ISSR markers
RESUMO
The growth hormone gene could be an attractive candidate gene for milk production in goats. Single-strand conformation polymorphism was used to identify polymorphism at the goat growth hormone [gGH] gene. For this purpose, genotyping of 90 Talli goat breeds was performed. Nine conformational patterns were observed in exon 4 of the gGH gene, with frequencies of 27.7% for the homozygous pattern [AA] and 72.2% for all of other heterozygous patterns [A/B, A/C, A/B/C, A/B/D/E, A/B/C/F, A/C/F, A/B/E, A/B/F]. The results showed that exon 4 of the GH gene in Talli goats is highly polymorphic