Protective role of ascorbic acid on population of -T- lymphocyte in major salivary glands of rat exposed to cadmium [immuno-histochemical study]

The study was carried out to evaluate the immunohistochemical investigation of the protective role of ascorbic acid on population of T-lymphocyte in major salivary glands of rat exposed to cadmium. Fifteen male rats weighting about 250g, were divided into three groups, 5 animals for each. In group I, the rats were injected with 0.5 ml of physiological saline and used as control. In group II, the animals were injected with cadmium chloride [15mg/kg body weight] for 1.4 and 8 weeks. In group III, the rats were injected with cadmium chloride simultaneously with ascorbic acid for different periods equal to the periods cadmium chloride injection. The results demonstrated that, by using of the anti-CD3 monoclonal antibody, the immunohistochemical finding of group II demonstrated weak positive staining with anti-CD3 antibody and a few number of positive T lymphocyte was situated in the connective tissue storma, as well as, parenchymal tissue of salivary glands. In group III, strong positive reactivity with anti-CD3 antibody in all examined tissue. There was great number of anti-CD3 positive T-lymphocyte scattered in the glandular tissue than that seen in group II. The ascorbic acid demonstrated beneficial effect on the cadmium toxicity exposed salivary gland when both drugs were simultaneously administrated and toxic effect of cadmium on the immune system can be obliterated by a sufficient intake of ascorbic acid. We conclude that immune functional abnormalities can be restored after toxic chemicals exposure by oral usage of ascorbic acid

influence of testosterone administration on the partially extirpated submandibular salivary gland in rat

This study was carried out in order to investigate the effect of testosterone administration on the regenerative power of the extirpated submandibular gland of rats. Thirty two Spargue-Dawley rats weighting about 240 gm were divided into two groups, 16 animals for each. In group 1 about half of right submandibular gland was removed and the animals were injected intraperitoneally at five successive days by testosterone. At different intervals [1,7,15 and 30 days] four rats were sacrified. Morphological and histochemical studies revealed healing of the extirpated area occured as a result of regeneration of secretory part by formation of newly formed serous acini from the old ones at the line of section and from rudimentary ducts in the central area. In the central area, the periductal cells were transformed into acinar cells and later, some of the straited ducts changed further to form granular convoluted tubules. In our study, group II revealed progressive newly formed acini and ducts. Some of these ducts demonstrated apical vacuolization within the first period, which could be considered as earlier sign of transformation into granular convoluted tubules, which appeared in later period in control groups. The lumina of these ducts appeared with eosinophilic products, which might indicate either that lumina carry acinar secretions or producing such material. The acini of group II became larger and its cytoplasm appeared as honeycombed appearance due to fusion of the secretory granules together. The appearance of the mucous cells or acini in group II may arise either from the proliferation of the already found mucous cells or as a result of sexual maturity. The newly formed acini showed strong PAS reaction, which decreased with maturation, denoting increase in the metabolic activity. The study has demonstrated that after testosterone administration the regenerative process of the submandibular gland was accelerated

Role of T and B lymphocytes in spontaneous lymphocyte proliferation in chronic inflammatory periodontal disease

Spontaneous lymphocyte responses of whole mononuclear cells [MNCs, T-lymphocytes and B-lymphocyte in patients with periodontal disease and theircontrols were studied in this work utilizing the tritiated thymidine uptakeassay. The periodontitis patients exhibited lower lymphoproliferativeresponse of MNCs compared with the controls. However, theB-lymphoproliferative response of patients showed the highest proliferativeresponse compared with that of all cell types studied. This finding maysuggest that the elimination of T-cell affects the hyperresponsiveness ofB-lymphocyte state

Specific demonstration of actin filaments in salivary gland tumors using immunohistochemistry

Neoplastic and non-neoplastic salivary gland tumors represented by pleomorphicadenoma and chronic sclerosing sialadenitis were selected for theirsimilarities to the normal structural elements of salivary glands, i.e. thepresence of developed duct-like pattern. The tissues were investigated bymeans of immunocytochemistry using antibodies against the filaments of actinin paraffin-embedded material. Actin was demonstrated in tumor cells in a waycorrelated to the topographic distribution of actin-rich cells, i.e. themyoepithelial cells. Also, the identification of such type of cells providesmore information about the proliferating nature of these myoepithelial cellsand its role in the histogenesis of salivary gland tumors

Development of human minor sublingual gland: a histological and histochemical study

The histological and histochemical changes occurring during the development of the human minor sublingual gland was studied. Tissue specimens including the tongue and floor of the mouth, obtained from 35 normal fetus of both sexes, were fixed in 10% formalin. Fixed tissues were routinely prepared, serially sectioned, and stained with H and E, bromphenol blue, PAS with or without diastase, and PAS and AB with and without sulphation, respectively. The results demonstrated that the gland was developed at about 12 WIU, from collections of epithelial basal cells of sublingual fold of the floor of the mouth that form a primary laminae. These were followed by cavitation and formation of excretory ducts. By age, the excretory ducts arborized to form the collecting ducts and arborized further to form the intercalated ducts that terminate in mucous acini. The gland reached maturity long before birth. In mature glands, the major secretory component in both mucous acini and mucous cells of collecting ducts was formed of sulfated acidic glucoprotein, while the goblet cells showed non sulfated type

Connective tissue response to tetracycline-incorporated resins

This experimental study was undertaken to determine the response of connective tissue to both self-cured and visible light cured resin containing 10% [w: w] tetracycline hydrochloride. The visible light cured resin considered the most compatible material. Marked reduction in the degree of inflammatory cell infiltration in both capsular and pericapsular tissues and increased degree of fibrous elements maturity of the fibrous capsule were noted when tetracycline was added to the test materials, during the whole observable periods

effect of metronidazole on surcutaneous tissues in rats

An attention was paid to evaluate the effect of implants visible light cured resin, and self-cured acrylic resin containing 10% metronidazole, upon the rat connective tissue. The best connective tissue response was obtained when metronidazole was added to self-cured acrylic resin, compared with other tested materials. It can be concluded that some antimicrobial agents can be added beneficially to acrylic resins used in dentistry as means of inflammation reduction, as well as to promote healing