Significant associations of the rs3104413 single-nucleotide polymorphism in the hla region with type 1 diabetes

Background and Aims: In this study, the effect of rs310441 polymorphism in the human leukocyte antigen [HLA] region on the development of susceptibility or resistance to Type 1 diabetes [T1D] among the people with T1D compared to healthy subjects has been investigated

Materials and Methods: This research, which is based on the examination of 130 cases with T1D and 98 controls, has been carried out in the city of Hamedan after clinical examination. In order to determine the HLA gene polymorphism, the allele-specific-refractory mutation system-polymerase chain reaction [ARMS-PCR] method was utilized

Results: This study indicated that there is a significant relationship between the frequency of alleles and genotypes in the patients compared to healthy subjects. The C/C and C/G genotypes were more frequent in patients than controls and G/G genotype was shown to be protective for T1D [p=0.01]. Significant difference was found for the G allelic frequency in patients with T1D and in the control group. The allelic frequency was significantly different between the two groups [p=0.0001]. Our findings indicate that HLA polymorphism[C/G] and [C/C] genotypes could be considered as genetic risk factors associated with susceptibility and [G/G] genotypes associated with protection for T1D

Conclusions: This study identified that there is a significant relationship between the frequency of alleles and genotypes in the patients compared to healthy subjects

Association between HLA-E gene polymorphism and unexplained recurrent spontaneous abortion [RSA] in Iranian women

Background: Human leukocyte antigen-E [HLA-E]is a non-classical major histocompatibility complex [MHC] class I antigens which expressed on extra villous cytotrophoblast, which interacts with NKG2A, is an inhibitory receptor on natural killer [NK] cells and leading to down regulation of immune response in the maternal-fetal interface and provides maternal immune tolerance of the fetus

Objective: This study was designated to investigate the gene frequencies of E0101 and E0103 in HLA-E gene in Iranian women with recurrent spontaneous abortion [RSA]

Materials and Methods: Amplification Refractory Mutation System [ARMS-PCR] technique was carried out to detect polymorphism in exon 3 of the HLA-E gene in women with RSA and controls [n=200]. Differences between groups were analyzed by SPSS19 software using X[2] test

Results: There was no significant difference in the allele frequencies of the HLA-E polymorphism between RSA and fertile controls but HLA-E 0101/0103 heterozygous genotype was found to be significantly higher in RSA group [p=0.006, OR=1.73], so this genotype might confer susceptibility to RSA

Conclusion: Our results suggest that HLA-E 0101/0103 heterozygous genotype leads to increase of RSA risk. It seems that by genotyping of HLA-E polymorphism, we can predict the risk of RSA in infertile women

Serum levels of IL-17A increase in asthma but don't correlate with serum level of IgE and asthma severity

Background and Aims: recent evidence suggests that T helper 17 [Th17] cells are involved in the emergence of asthma. Th17 cells have a key role in inducing inflammation in asthmatic airways. Thus interleukin [IL]-17A, the main cytokine of Th17, contributes to airways inflammation

Materials and Methods: we evaluated the level of IL-17A and total immunoglobulin E [IgE] by ELISA method in sera of 100 asthmatic patients and 81 healthy controls, to determine if serum concentration of IL-17A is associated with asthma severity. We classified patients into three groups; mild [n=28], moderate [n=33] and severe asthma [n=39]

Results: serum IL-17A and IgE concentrations were significantly higher in the asthmatic patients than the control group [p=0.026 and p<0.01, respectively]. Mean serum IL-17A and IgE values were 37.73 pg/ml and 39.02 IU/ml in the control group, but 68.55 pg/ml and 295.87 IU/ml in the patients group, respectively. Nevertheless, there were no significant differences between the three groups of asthmatic patients. Mean serum IL-17A and IgE values were 94.17 pg/ml and 255.07 IU/ml in the mild group, 71.29 pg/ml and 271.27 IU/ml in the moderate group, and 47.85 pg/ml and 345.97 IU/ml in the severe group, respectively. Moreover, there was no correlation between serum levels of IL-17A and IgE

Conclusion: it was found that IL-17A, like IgE, rises in sera of asthmatic patients though in a different manner. IgE increases in serum consistent with disease severity though the increase of IL-17A in serum has an inverse relationship with IgE elevation