Spectrophotometric method for the determination of Gemifloxacin mesylate in pure and tablet dosage form

A Spectrophotometric method for the determination of Gemifloxacin mesylate [GFX] is developed and validated according to ICH guidelines. GFX is a fluoroquinolone that is used in the treatment of pneumonia. The analysis of the pure drug was carried out at its 'k[max] 270 nm. The method was linear from 0.5-5microg/mL, r[2] 0.999 and equation is 0.102-0.000. The % RSD for inter-day [0.969%] and intra-day [0.714%] assuring a good precision and accuracy was close to 100%. Limit of detection and Limit of quantification were 0.197 and 0.599|ug/mL, respectively

The validation results and statistical data demonstrate that the method is accurate, sensitive, cost effective and reproducible and has an importance in quality assurance of GFX analysis. The developed method was proved suitable for analysis of GFX in the pure and tablet dosage forms without interference of excepients

Incidence of tuberculosis in cervical lymphadenopathy

Objectives: To find out the incidence of tuberculosis in cervical lymphadenopathy and patients characteristic

Study Design: Descriptive study

Place and Duration: This study carried out in surgical unit I Civil Hospital Jacobabad from January 2009 to December 2011

Methodology: Patients having cervical lymphadenopathy above the age of 12 years of both sexes were included in this study. Fine needle aspiration cytology [FNAC] of lymph nodes was done, and excision biopsy was also performed when ever required, apart from that complete history, physical examination ESR and other required haemotological test performed

Results: A total number of 180 patients with enlarge lymph nodes both male female adults were included, 68.88% [n=124] were below the age of 30 years, remaining 32.22% [n=56] were above this age group, 76.11% [n=137], patients had tuberculosis lymphadenitis. In 11.66% patients [n=21] reactive hyperplasia, 8.88% patients [n=16], chronic non specific lymphadenitis, 2.77% patient [n=05] lymphoma and 0.55% patient [n=1] metastasis to cervical lymph nodes were diagnosed. FNAC was conclusive in 87.77%, while ESR was raised in 26.66% cases. Constitutional symptoms were not significant, majority [n=119] of patients belong to poor class

Conclusion: Tuberculosis was most common diseases involving the cervical lymph nodes in both sexes who belong to poor socio economical class. Haemotological findings were inconclusive in majority of patients the FNAC diagnosis highly conclusive but few required lymph node biopsy in few cases