[Evaluation of anti tumor activity of candida albicans cell wall fraction on human fibrosarcoma cell in vitro]

Matrix metalloproteinases [MMPs] plays a role in the several physiologic and pathologic events. There is some evidence indicating the involvement of MMPs in tumor invasion and inflammatory diseases. In this study we evaluated the effect of cell wall Candida albicans fraction on MMP production by the fibrosarcoma cell line using an in vitro cytotoxicity assay, sodium dodecyl sulfate-polyacrylamide, and gelatin zymography. It was an experimental study. Fraction of cell wall Candida albicans was extracted by lysis buffer and] 0, 10, 50, 100 [micro g/ml from fraction was added to human fibrosarcoma cell line in 96 well microplate and inhibitory effect on tumor cell invasion was investigated by MTT and zymography test. Our results showed that fraction of cell wall Candida albicans was found to exhibit a inhibitory effect on tumor cell line in MTT test because the viability of tumor cells was decreased versus the control groups. Also the production of metalloproteinases enzyme in presence of fraction was diminished significantly [P<0.05]. Since inhibition of MMP activity has been employed in modality therapy in diseases such as cancer, this fraction could be promised in the preparation of anti-MMP therapeutic derivatives

Molecular epidemiology of Staphylococcus aureus isolated from patients and personnel in army hospital

Staphylococcus aureus has emerged over the past several decades as a leading cause of hospital- and community acquired infection. A significant component in the success has been its acquisition of antibiotic Resistant factors. We have developed a rapid and simplified approach for the strain characterization of staph.aureus on the basis of multilocussequence typing. MLRFT for s.aureus involes amplification of seven house keeping gene locus, the amplicons are then digested directly with one or two restriction Enzyme and the restriction fragments are resolved by agarose gel electrophoresis. These isolates had been additionally characterized for their susceptibilities to antibiotics. MLRFT resolved 146 isolates into 19 RFT that 48 isolates have the same molecular patterns. Their susceptibilities to antibiotics were showed 90% resistance to methicillin. MLRFT provides convenient procedures for molecular epidemiology it requires minimal laboratory facilities and is relatively simple and inexpensive to perform.63% of cluster [24 isolates] take placed two fold cluster that 90% of them was resistant to methicillin, 55% belong to twofold cluster, there fore methicillin resistant isolates can transmit easily

[Evaluation of toxicity chemical substances [extract oihyndropyridine derivatives, saff flower and peganum harmala] By saccharomyces cerevisiae]

Background: the toxicity of many chemical substances has been studied by many of living organisms used as biological indicators. Higher organisms[laboratory animals] are generally used but the response time may be lengthy. Never the less by exploiting uni cellular organisms, in particulary yeast, it is possible to reduce testing time considerably. In this study, the budding yeast, saccharomyces cerevisiae asimple non-pathogenic eukaryotic organisms, which is easy to cultivate, has been used as experimental model for assaying acute toxicity of chemical substances

Material and Methods: As a experimental study, The first step yeast cells were cultured in broth media and correlation cell of numbers and optical density was determined in 525 nm. Then, yeast cells were cultured in the presence of various concentration of chemical substances and cell density was measured. the proliferation rate determined from the logarithmic growth phase. A dose-response curve was obtained by plotting the relative growth rate versus the chemical concentration which concentrations leading to 50% inhibition of growth[IC50] was determined

Results: the results showed a concentration - dependent cytoxicity effect for saff flower extract [IC50=112[micro]lml-1] and peganum harmala extract [IC50=476[micro]gml-1]. The toxicity effects of dihidropyridine derivatives was studied up to 100 [micro]Mml-1, but no cytotoxic effect was observed

Conclusion: these results were agreement with other cytotoxic tests [i.e, rat, hamster, brin shrimp, and human cell cultures] then this procedure can be used as the first step, for assaying acute toxicity of chemical substances